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Literature summary extracted from

  • Wu, T.; Pei, J.; Ge, L.; Wang, Z.; Ding, G.; Xiao, W.; Zhao, L.
    Characterization of a alpha-L-rhamnosidase from Bacteroides thetaiotaomicron with high catalytic efficiency of epimedin C (2018), Bioorg. Chem., 81, 461-467 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expression in Escherichia coli Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 Cu2+ 5 mM, 92% loss of activity. 1 mM, 83% loss of activity Bacteroides thetaiotaomicron
3.2.1.40 Fe2+ 5 mM, 12% loss of activity Bacteroides thetaiotaomicron
3.2.1.40 Fe3+ 5 mM, 12% loss of activity Bacteroides thetaiotaomicron
3.2.1.40 Hg2+ 1 mM, 99% loss of activity Bacteroides thetaiotaomicron
3.2.1.40 Ni2+ 5 mM, 28% loss of activity Bacteroides thetaiotaomicron
3.2.1.40 Zn2+ 5 mM, 32% loss of activity. 1 mM, 55% loss of activity Bacteroides thetaiotaomicron

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 2.87
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 55°C Bacteroides thetaiotaomicron

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.40 Ca2+ 5 mM, 1.3fold activation Bacteroides thetaiotaomicron
3.2.1.40 Mn2+ 5 mM, 1.26fold activation Bacteroides thetaiotaomicron

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 83400
-
 SDS-PAGE Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Bacteroides thetaiotaomicron Q8A904
-
-
3.2.1.40 Bacteroides thetaiotaomicron VPI-5482 Q8A904
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside Bacteroides thetaiotaomicron 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O no activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside Bacteroides thetaiotaomicron VPI-5482 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 epimedin C + H2O
-
 Bacteroides thetaiotaomicron icariin + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 epimedin C + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 icariin + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Bacteroides thetaiotaomicron hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 additional information no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.40 additional information no the enzyme exhibits high selectivity to cleave the alpha-1,2 and alpha-1,6 glycosidic bond between rhamnoside and rhamnoside, rhamnoside and glycoside Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
3.2.1.40 rutin + H2O
-
 Bacteroides thetaiotaomicron isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Bacteroides thetaiotaomicron VPI-5482 isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 ? x * 83400, SDS-PAGE Bacteroides thetaiotaomicron

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 BtRha
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 55
-
 hydrolysis of epimedin C, hydrolysis of 4-nitrophenyl alpha-L-rhamnopyranoside Bacteroides thetaiotaomicron

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 40 60 40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity, hydrolysis of 4-nitrophenyl alpha-L-rhamnopyranoside Bacteroides thetaiotaomicron
3.2.1.40 50 60 50°C: about 65% of maximal activity, 60°C: about 75% of maximal activity, hydrolysis of epimedin C Bacteroides thetaiotaomicron

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 45
-
 4 h, residual activity is more than 50% Bacteroides thetaiotaomicron
3.2.1.40 50
-
 half-life: 1 h Bacteroides thetaiotaomicron

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 1743.29
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 55°C Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 6.5
-
-
 Bacteroides thetaiotaomicron

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 5 8 stable in the pH range 5.0-8.0 at 45°C for 4 h over 60% Bacteroides thetaiotaomicron

Expression

EC Number Organism Comment Expression
3.2.1.40 Bacteroides thetaiotaomicron the specific activity of rhamnosidase was 0.57 U/mg in LB medium with 0.1 mM isopropyl beta-D-thiogalactoside induction at 28 °C for 8 h up