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Literature summary extracted from

  • Silchenko, A.S.; Rasin, A.B.; Zueva, A.O.; Kusaykin, M.I.; Zvyagintseva, T.N.; Kalinovsky, A.I.; Kurilenko, V.V.; Ermakova, S.P.
    Fucoidan sulfatases from marine bacterium Wenyingzhuangia fucanilytica CZ1127T (2018), Biomolecules, 8, 98.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.B2 expression in Escherichia coli Wenyingzhuangia fucanilytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.6.B2 Ca2+ the enzyme exhibits catalytic activity only in the presence of Ca2+ ions Wenyingzhuangia fucanilytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.B2 55000
-
 SDS-PAGE Wenyingzhuangia fucanilytica
3.1.6.B2 55940
-
 calculated from sequence Wenyingzhuangia fucanilytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O Wenyingzhuangia fucanilytica the enzyme catalyzes a necessary step for subsequent fucoidan degradation by fucosidases alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O Wenyingzhuangia fucanilytica CZ1127 the enzyme catalyzes a necessary step for subsequent fucoidan degradation by fucosidases alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.B2 Wenyingzhuangia fucanilytica A0A1B1Y5P4
-
-
3.1.6.B2 Wenyingzhuangia fucanilytica CZ1127 A0A1B1Y5P4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.B2 one step purification on Ni-NTA resin Wenyingzhuangia fucanilytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.B2 4-nitrophenyl sulfate + H2O
-
 Wenyingzhuangia fucanilytica 4-nitrophenol + sulfate
-
 ?
3.1.6.B2 4-nitrophenyl sulfate + H2O
-
 Wenyingzhuangia fucanilytica CZ1127 4-nitrophenol + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O
-
 Wenyingzhuangia fucanilytica alpha-L-Fucp-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O
-
 Wenyingzhuangia fucanilytica CZ1127 alpha-L-Fucp-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O
-
 Wenyingzhuangia fucanilytica alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O
-
 Wenyingzhuangia fucanilytica alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O the enzyme catalyzes a necessary step for subsequent fucoidan degradation by fucosidases Wenyingzhuangia fucanilytica alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O
-
 Wenyingzhuangia fucanilytica CZ1127 alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + H2O the enzyme catalyzes a necessary step for subsequent fucoidan degradation by fucosidases Wenyingzhuangia fucanilytica CZ1127 alpha-L-Fucp-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) + sulfate
-
 ?
3.1.6.B2 additional information the enzyme has no action on native fucoidans from Fucus evanescens or Sargassum horneri. It does not catalyse desulfation of oligosaccharides alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-), alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) and alpha-L-Fucp-(1->2)-alpha-L-Fucp-(1->4)-[alpha-L-Fucp(2,3-diOSO3-)-(1->3)]-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) Wenyingzhuangia fucanilytica ?
-
 ?
3.1.6.B2 additional information the enzyme has no action on native fucoidans from Fucus evanescens or Sargassum horneri. It does not catalyse desulfation of oligosaccharides alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-), alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2,4-diOSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) and alpha-L-Fucp-(1->2)-alpha-L-Fucp-(1->4)-[alpha-L-Fucp(2,3-diOSO3-)-(1->3)]-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2,3-diOSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) Wenyingzhuangia fucanilytica CZ1127 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.B2 sulfated fucoidan exo-2O-sulfatase
-
 Wenyingzhuangia fucanilytica
3.1.6.B2 Swf1
-
 Wenyingzhuangia fucanilytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.B2 40 45
-
 Wenyingzhuangia fucanilytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.B2 7.8 8.4
-
 Wenyingzhuangia fucanilytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.B2 6 9 catalytically active over a wide pH range from 6.0 to 9.0 Wenyingzhuangia fucanilytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.6.B2 Wenyingzhuangia fucanilytica calculated from sequence
-
 8.25