BRENDA - Enzyme Database

Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug

Tao, X.; Jia, N.; Cheng, N.; Ren, Y.; Cao, X.; Liu, M.; Wei, D.; Wang, F.Q.; Biomaterials 131, 1-14 (2017)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.4.4
medicine
design of a safe and simple amphiphilic prodrug delivery system, based on the elevated expression of phospholipase D in cancer cells. The method utilizes the transphosphatidylation ability of bacterial PLD on alcohol groups and the hydrolysis activity of overexpressed PLD on phospholipids in cancer cells. Phosphatidyl-doxorubicin is synthesized by bacterial PLD. The prodrug can be selfassembled to nanoparticles with uniform size and doxorubicin can be selectively released in cancer cells triggered by the locally overexpressed PLD. The method can significantly prolong the half-life of doxorubicin in the tumors and decrease the distribution in heart and kidney
Homo sapiens
3.1.4.4
medicine
design of a safe and simple amphiphilic prodrug delivery system, based on the elevated expression of phospholipase D in cancer cells. The method utilizes the transphosphatidylation ability of bacterial PLD on alcohol groups and the hydrolysis activity of overexpressed PLD on phospholipids in cancer cells. Phosphatidyl-doxorubicin is synthesized by bacterial PLD. The prodrug can be selfassembled to nanoparticles with uniform size and doxorubicin can be selectively released in cancer cells triggered by the locally overexpressed PLD. The method can significantly prolong the half-life of doxorubicin in the tumors and decrease the distribution in heart and kidney
Streptomyces chromofuscus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Homo sapiens
-
-
-
3.1.4.4
Streptomyces chromofuscus
Q8KRU5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
doxorubicin + phosphatidate
-
750001
Streptomyces chromofuscus
phosphatidyl-doxorubicin + H2O
-
-
-
?
3.1.4.4
phosphatidyl-doxorubicin + H2O
-
750001
Homo sapiens
doxorubicin + phosphatidate
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.4.4
medicine
design of a safe and simple amphiphilic prodrug delivery system, based on the elevated expression of phospholipase D in cancer cells. The method utilizes the transphosphatidylation ability of bacterial PLD on alcohol groups and the hydrolysis activity of overexpressed PLD on phospholipids in cancer cells. Phosphatidyl-doxorubicin is synthesized by bacterial PLD. The prodrug can be selfassembled to nanoparticles with uniform size and doxorubicin can be selectively released in cancer cells triggered by the locally overexpressed PLD. The method can significantly prolong the half-life of doxorubicin in the tumors and decrease the distribution in heart and kidney
Homo sapiens
3.1.4.4
medicine
design of a safe and simple amphiphilic prodrug delivery system, based on the elevated expression of phospholipase D in cancer cells. The method utilizes the transphosphatidylation ability of bacterial PLD on alcohol groups and the hydrolysis activity of overexpressed PLD on phospholipids in cancer cells. Phosphatidyl-doxorubicin is synthesized by bacterial PLD. The prodrug can be selfassembled to nanoparticles with uniform size and doxorubicin can be selectively released in cancer cells triggered by the locally overexpressed PLD. The method can significantly prolong the half-life of doxorubicin in the tumors and decrease the distribution in heart and kidney
Streptomyces chromofuscus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
doxorubicin + phosphatidate
-
750001
Streptomyces chromofuscus
phosphatidyl-doxorubicin + H2O
-
-
-
?
3.1.4.4
phosphatidyl-doxorubicin + H2O
-
750001
Homo sapiens
doxorubicin + phosphatidate
-
-
-
?