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Literature summary extracted from
- Engineering Pseudomonas putida KT2440 for simultaneous degradation of organophosphates and pyrethroids and its application in bioremediation of soil (2015), Biodegradation, 26, 223-233 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | agriculture | agricultural soils are often co-contaminated with different types of pesticides. For example, organophosphates and pyrethroids usually are applied together for pest control. So far, microorganisms with both OP- and pyrethroid-degrading activity have not been isolated from natural environments. Alternatively, construction of engineered microbes with broad-spectrum pesticide-degrading activity may be a promising strategy for bioremediation of mixed pesticides-contaminated soil. To construct multifunctional pesticide degrading microorganisms, the co-expression of multiple degrading enzymes in a host strain may be a feasible approach. Both MPH and PytH are coexpressed in a soil bacterium Pseudomonas putida KT2440, resulting in an engineered strain with the capability to simultaneously degrade OPs and pyrethroids. Six pesticides (methyl parathion, fenitrothion, chlorpyrifos, permethrin, fenopropathrin, cypermethrin) can be effectively degraded by the engineered Pseudomonas putida KT2440. Therefore, the engineered Pseudomonas putida KT2440 could potentially be applied for in situ bioremediation of soil co-contaminated with organophosphates and pyrethroids | Sphingobium wenxiniae |
| 3.1.8.1 | agriculture | agricultural soils are often co-contaminated with different types of pesticides. For example, organophosphates and pyrethroids usually are applied together for pest control. So far, microorganisms with both OP- and pyrethroid-degrading activity have not been isolated from natural environments. Alternatively, construction of engineered microbes with broad-spectrum pesticide-degrading activity may be a promising strategy for bioremediation of mixed pesticides-contaminated soil. To construct multifunctional pesticide degrading microorganisms, the co-expression of multiple degrading enzymes in a host strain may be a feasible approach. Both MPH and PytH are coexpressed in a soil bacterium Pseudomonas putida KT2440, resulting in an engineered strain with the capability to simultaneously degrade OPs and pyrethroids. Six pesticides (methyl parathion, fenitrothion, chlorpyrifos, permethrin, fenopropathrin, cypermethrin) can be effectively degraded by the engineered Pseudomonas putida KT2440. Therefore, the engineered Pseudomonas putida KT2440 could potentially be applied for in situ bioremediation of soil co-contaminated with organophosphates and pyrethroids | Stenotrophomonas sp. YC-1 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.88 | functional expression of mpd and pytH in Pseudomonas putida KT2440 under the control of a constitutive BioBrick promoter J23119 | Sphingobium wenxiniae |
| 3.1.8.1 | functional expression of mpd and pytH in Pseudomonas putida KT2440 under the control of a constitutive BioBrick promoter J23119 | Stenotrophomonas sp. YC-1 |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.88 | 31000 | - |
PytH-His6 fusion protein, SDS-PAGE | Sphingobium wenxiniae |
| 3.1.8.1 | 32000 | - |
SDS-PAGE | Stenotrophomonas sp. YC-1 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.88 | Sphingobium wenxiniae | C0LA90 | - |
- |
| 3.1.1.88 | Sphingobium wenxiniae DSM 21828 | C0LA90 | - |
- |
| 3.1.8.1 | Stenotrophomonas sp. YC-1 | Q0PWQ4 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.88 | cypermethrin + H2O | - |
Sphingobium wenxiniae | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? |  |
| 3.1.1.88 | cypermethrin + H2O | - |
Sphingobium wenxiniae DSM 21828 | 3-phenoxybenzaldehyde + 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide | - |
? | |
| 3.1.1.88 | fenopropathrin + H2O | - |
Sphingobium wenxiniae | 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide | - |
? | |
| 3.1.1.88 | fenopropathrin + H2O | - |
Sphingobium wenxiniae DSM 21828 | 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide | - |
? | |
| 3.1.1.88 | permethrin + H2O | - |
Sphingobium wenxiniae | 3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate | - |
? | |
| 3.1.1.88 | permethrin + H2O | - |
Sphingobium wenxiniae DSM 21828 | 3-phenoxybenzyl alcohol + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate | - |
? | |
| 3.1.8.1 | chlorpyrifos + H2O | - |
Stenotrophomonas sp. YC-1 | ? | - |
? | |
| 3.1.8.1 | fenitrothion + H2O | - |
Stenotrophomonas sp. YC-1 | ? | - |
? | |
| 3.1.8.1 | methyl parathion + H2O | - |
Stenotrophomonas sp. YC-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | ? | x * 31000, PytH-His6 fusion protein, SDS-PAGE | Sphingobium wenxiniae |
| 3.1.8.1 | ? | x * 32000, SDSD-PAGE | Stenotrophomonas sp. YC-1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.88 | pytH | - |
Sphingobium wenxiniae |
| 3.1.8.1 | methyl parathion hydrolase | - |
Stenotrophomonas sp. YC-1 |
| 3.1.8.1 | Mph | - |
Stenotrophomonas sp. YC-1 |
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