BRENDA - Enzyme Database

Characterization of a new alpha-L-fucosidase isolated from Fusarium proliferatum LE1 that is regioselective to alpha-(1->4)-L-fucosidic linkage in the hydrolysis of alpha-L-fucobiosides

Shvetsova, S.V.; Shabalin, K.A.; Bobrov, K.S.; Ivanen, D.R.; Ustyuzhanina, N.E.; Krylov, V.B.; Nifantiev, N.E.; Naryzhny, S.N.; Zgoda, V.G.; Eneyskaya, E.V.; Kulminskaya, A.A.; Biochimie 132, 54-65 (2017)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-deoxyfuconojirimycin
-
Fusarium proliferatum
3.2.1.51
alpha-L-fucose
competitive
Fusarium proliferatum
3.2.1.51
Tris(hydroxymethyl)aminomethane
-
Fusarium proliferatum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.53
-
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.1
-
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.23
-
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.67
-
propyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
3
-
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Cu2+
2 mM, enzyme retains 38% of its activity
Fusarium proliferatum
3.2.1.51
Hg2+
2 mM, enzyme retains 25% of its activity
Fusarium proliferatum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
67000
-
SDS-PAGE
Fusarium proliferatum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Fusarium proliferatum
A0A1L3GZ76
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
-
Fusarium proliferatum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + GlcNAc-poly-acrylamide polymer
-
-
-
?
3.2.1.51
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
additional information
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
749983
Fusarium proliferatum
?
-
-
-
-
3.2.1.51
propyl alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
propanol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 67000, SDS-PAGE
Fusarium proliferatum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
45
-
-
Fusarium proliferatum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.2.1.51
37
60
37C: about 50% of maximal activity, 60C: about 25% of maximal activity
Fusarium proliferatum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
50
-
20 min, 55% loss of activity
Fusarium proliferatum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.51
39.8
-
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
-
Fusarium proliferatum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
4
6.5
pH 4.0: about 60% of maximal activity, pH 6.5: about 70% of maximal activity
Fusarium proliferatum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
-
activity rapidly decreases below pH 4.0
Fusarium proliferatum
3.2.1.51
5
6
stable
Fusarium proliferatum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.2
-
alpha-L-fucose
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
7.1
-
1-deoxyfuconojirimycin
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
12.2
-
Tris(hydroxymethyl)aminomethane
pH 5.5, 37C
Fusarium proliferatum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.51
Fusarium proliferatum
2D-electrophoresis
-
5.21
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-deoxyfuconojirimycin
-
Fusarium proliferatum
3.2.1.51
alpha-L-fucose
competitive
Fusarium proliferatum
3.2.1.51
Tris(hydroxymethyl)aminomethane
-
Fusarium proliferatum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.2
-
alpha-L-fucose
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
7.1
-
1-deoxyfuconojirimycin
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
12.2
-
Tris(hydroxymethyl)aminomethane
pH 5.5, 37C
Fusarium proliferatum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.53
-
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.1
-
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.23
-
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
1.67
-
propyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
3.2.1.51
3
-
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Cu2+
2 mM, enzyme retains 38% of its activity
Fusarium proliferatum
3.2.1.51
Hg2+
2 mM, enzyme retains 25% of its activity
Fusarium proliferatum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
67000
-
SDS-PAGE
Fusarium proliferatum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
-
Fusarium proliferatum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
-
749983
Fusarium proliferatum
alpha-L-fucose + GlcNAc-poly-acrylamide polymer
-
-
-
?
3.2.1.51
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
additional information
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
749983
Fusarium proliferatum
?
-
-
-
-
3.2.1.51
propyl alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
propanol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
3.2.1.51
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside + H2O
-
749983
Fusarium proliferatum
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 67000, SDS-PAGE
Fusarium proliferatum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
45
-
-
Fusarium proliferatum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.2.1.51
37
60
37C: about 50% of maximal activity, 60C: about 25% of maximal activity
Fusarium proliferatum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
50
-
20 min, 55% loss of activity
Fusarium proliferatum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.51
39.8
-
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37C
Fusarium proliferatum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
-
Fusarium proliferatum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
4
6.5
pH 4.0: about 60% of maximal activity, pH 6.5: about 70% of maximal activity
Fusarium proliferatum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
-
activity rapidly decreases below pH 4.0
Fusarium proliferatum
3.2.1.51
5
6
stable
Fusarium proliferatum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.51
Fusarium proliferatum
2D-electrophoresis
-
5.21