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Literature summary extracted from

  • Shvetsova, S.V.; Shabalin, K.A.; Bobrov, K.S.; Ivanen, D.R.; Ustyuzhanina, N.E.; Krylov, V.B.; Nifantiev, N.E.; Naryzhny, S.N.; Zgoda, V.G.; Eneyskaya, E.V.; Kulminskaya, A.A.
    Characterization of a new alpha-L-fucosidase isolated from Fusarium proliferatum LE1 that is regioselective to alpha-(1->4)-L-fucosidic linkage in the hydrolysis of alpha-L-fucobiosides (2017), Biochimie, 132, 54-65 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 1-deoxyfuconojirimycin
-
 Fusarium proliferatum
3.2.1.51 alpha-L-fucose competitive Fusarium proliferatum
3.2.1.51 Tris(hydroxymethyl)aminomethane
-
 Fusarium proliferatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.53
-
 propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 1.1
-
 4-nitrophenyl alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 1.23
-
 propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 1.67
-
 propyl alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 3
-
 propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Cu2+ 2 mM, enzyme retains 38% of its activity Fusarium proliferatum
3.2.1.51 Hg2+ 2 mM, enzyme retains 25% of its activity Fusarium proliferatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 67000
-
 SDS-PAGE Fusarium proliferatum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Fusarium proliferatum A0A1L3GZ76
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Fusarium proliferatum 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.51 6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
-
 Fusarium proliferatum alpha-L-fucose + N,N'-diacetylchitobiose
-
 ?
3.2.1.51 alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
-
 Fusarium proliferatum alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
 ?
3.2.1.51 alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
-
 Fusarium proliferatum alpha-L-fucose + GlcNAc-poly-acrylamide polymer
-
 ?
3.2.1.51 beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
-
 Fusarium proliferatum ?
-
 ?
3.2.1.51 additional information the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors Fusarium proliferatum ?
-
 ?
3.2.1.51 propyl alpha-L-fucopyranoside + H2O
-
 Fusarium proliferatum propanol + alpha-L-fucopyranose
-
 ?
3.2.1.51 propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
-
 Fusarium proliferatum ?
-
 ?
3.2.1.51 propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
-
 Fusarium proliferatum ?
-
 ?
3.2.1.51 propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside + H2O
-
 Fusarium proliferatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 67000, SDS-PAGE Fusarium proliferatum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 FpFucA
-
 Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 45
-
-
 Fusarium proliferatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.51 37 60 37°C: about 50% of maximal activity, 60°C: about 25% of maximal activity Fusarium proliferatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 50
-
 20 min, 55% loss of activity Fusarium proliferatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 39.8
-
 4-nitrophenyl alpha-L-fucopyranoside pH 5.5, 37°C Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.5
-
-
 Fusarium proliferatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 4 6.5 pH 4.0: about 60% of maximal activity, pH 6.5: about 70% of maximal activity Fusarium proliferatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 4
-
 activity rapidly decreases below pH 4.0 Fusarium proliferatum
3.2.1.51 5 6 stable Fusarium proliferatum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 0.2
-
 alpha-L-fucose pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 7.1
-
 1-deoxyfuconojirimycin pH 5.5, 37°C Fusarium proliferatum
3.2.1.51 12.2
-
 Tris(hydroxymethyl)aminomethane pH 5.5, 37°C Fusarium proliferatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.51 Fusarium proliferatum 2D-electrophoresis
-
 5.21