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Literature summary extracted from

  • Gaber, Y.; Mekasha, S.; Vaaje-Kolstad, G.; Eijsink, V.G.H.; Fraaije, M.W.
    Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca (2016), Biochim. Biophys. Acta, 1864, 1253-1259 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Escherichia coli BL21(DE3) CodonPlus cells Thermobifida fusca
3.2.1.200 expressed in Escherichia coli BL21(DE3) cells Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 colloidal chitin substrate inhibition Thermobifida fusca
3.2.1.200 colloidal chitin substrate inhibition Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Thermobifida fusca
-
-
-
3.2.1.200 Thermobifida fusca Q47RL1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 HisTrap column chromatography and Superdex 75 gel filtration Thermobifida fusca
3.2.1.200 Ni-NTA column chromatography and Superdex 75 gel filtration Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 alpha-chitin + H2O
-
 Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 beta-chitin + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.14 chitohexaose + H2O
-
 Thermobifida fusca chitobiose + chitotetraose + chitotriose
-
 ?
3.2.1.14 additional information activity towards colloidal chitin and acetylated chitosan is minimal. The enzyme does not use N,N',N''-triacetyl-beta-D-chitotriose and RNase B as substrates Thermobifida fusca ?
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
-
 Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
-
 Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
-
 Thermobifida fusca 2 N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.200 alpha-chitin + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.200 beta-chitin + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.200 colloidal chitin + H2O minimal activity Thermobifida fusca ?
-
 ?
3.2.1.200 additional information no activity with N,N',N''-triacetylchitotriose Thermobifida fusca ?
-
 ?
3.2.1.200 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Thermobifida fusca N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
3.2.1.200 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Thermobifida fusca N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.200 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Thermobifida fusca 2 N,N'-diacetylchitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.200 ? x * 50000, SDS-PAGE Thermobifida fusca
3.2.1.200 ? x * 46300, calculated from amino acid sequence Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi18A
-
 Thermobifida fusca
3.2.1.14 GH18 chitinase
-
 Thermobifida fusca
3.2.1.200 GH18 chitinase
-
 Thermobifida fusca
3.2.1.200 Tfu0868
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40 45 at pH 6.5. At temperatures above 50 °C activity becomes drastically reduced Thermobifida fusca
3.2.1.200 40 45
-
 Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 57.5
-
 melting temperature Thermobifida fusca
3.2.1.200 57.5
-
 melting temperature Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6.5 7
-
 Thermobifida fusca
3.2.1.200 6.5 7
-
 Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 6 7.8 the enzyme displays more than 50% of maximum activity in the pH range of 6.0 to 7.8 Thermobifida fusca
3.2.1.200 6 7.8 the enzyme displays more than 50% of maximum activity in the pH range of 6.0 to 7.8, with a peak between 6.5 and 7.0 Thermobifida fusca