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Literature summary extracted from

  • Mai-Gisondi, G.; Maaheimo, H.; Chong, S.; Hinz, S.; Tenkanen, M.; Master, E.
    Functional comparison of versatile carbohydrate esterases from families CE1, CE6 and CE16 on acetyl-4-O-methylglucuronoxylan and acetyl-galactoglucomannan (2017), Biochim. Biophys. Acta, 1861, 2398-2405 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 expression in Pichia pastoris Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.72 additional information fusion of family 3 carbohydrate binding module CBM3 from Clostridium thermocellum to the carboxyl terminus of the acetyl xylan esterase. CBM3 fusion has negligible impact on the thermostability or regioselectivity of AXE, activities towards acetylated corncob xylan, 4-methylumbelliferyl acetate, 4-nitrophenyl acetate, and cellobiose octaacetate are also unchanged. The activity of the fusion protein on cellulose acetate is 4fold increased compared with wild-type Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.04
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 0.05
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 0.13
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 0.24
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Aspergillus nidulans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Aspergillus nidulans 4-methylumbelliferone + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans 4-nitrophenol + acetate
-
 ?
3.1.1.72 cellobiose octaacetate + H2O
-
 Aspergillus nidulans ? + acetate
-
 ?
3.1.1.72 cellulose acetate + H2O
-
 Aspergillus nidulans ? + acetate
-
 ?
3.1.1.72 corncob xylan + H2O
-
 Aspergillus nidulans ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 50
-
 half-life more than 48 h Aspergillus nidulans
3.1.1.72 60
-
 half-life 38.6 min Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.72 51
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 59
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 96
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 163
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.72 696
-
 4-methylumbelliferyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 726
-
 4-methylumbelliferyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 1310
-
 4-nitrophenyl acetate wild-type, pH 7.5, 50°C Aspergillus nidulans
3.1.1.72 1370
-
 4-nitrophenyl acetate fusion protein with CBM3, pH 7.5, 50°C Aspergillus nidulans