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Literature summary extracted from

  • Grant, G.A.
    Regulatory mechanism of Mycobacterium tuberculosis phosphoserine phosphatase SerB2 (2017), Biochemistry, 56, 6481-6490 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.3 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.3 D15A/E33A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
3.1.3.3 G18A/G108A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.3 L-serine L-serine is an allosteric feedback inhibitor that acts by decreasing the affinity of the substrate for the enzyme Mycobacterium tuberculosis
3.1.3.3 phosphate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.3 0.38
-
 O-phospho-L-serine at pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.3 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.3 O-phospho-D-serine + H2O Mycobacterium tuberculosis
-
 D-serine + phosphate
-
 ir
3.1.3.3 O-phospho-L-serine + H2O Mycobacterium tuberculosis
-
 L-serine + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.3 Mycobacterium tuberculosis O53289
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.3 O-phospho-D-serine + H2O
-
 Mycobacterium tuberculosis D-serine + phosphate
-
 ir
3.1.3.3 O-phospho-L-serine + H2O
-
 Mycobacterium tuberculosis L-serine + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.1.3.3 PSP
-
 Mycobacterium tuberculosis
3.1.3.3 SerB2
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.3 18.6
-
 O-phospho-L-serine at pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.3 0.019
-
 L-serine at pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.3 0.00078
-
 at pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis L-serine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.3 49000
-
 O-phospho-L-serine at pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis