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Literature summary extracted from

  • Miner, K.D.; Kurtz, D.M.
    Active site metal occupancy and cyclic di-GMP phosphodiesterase activity of Thermotoga maritima HD-GYP (2016), Biochemistry, 55, 970-979 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.52 expressed in Escherichia coli BL21pLysE (DE3) cells Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.52 D289A inactive Thermotoga maritima
3.1.4.52 E169A the mutant enzyme is active with 3',5'-di-cGMP Thermotoga maritima
3.1.4.52 H173A inactive Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.52 Iron accommodates di- and triiron active site Thermotoga maritima
3.1.4.52 Mn2+ contains Mn2+ Thermotoga maritima
3.1.4.52 additional information the enzyme can accommodate both di- and trimetal active sites. An as-isolated iron-containing TM0186 has an oxo/carboxylato-bridged diferric site, and the reduced diferrous form is necessary and sufficient to catalyze conversion of cyclic di-GMP to 5'-phosphoguanylyl(3',5')guanosine. Similar cyclic di-GMP phosphodiesterase activities are obtained with divalent iron or manganese. A Glu residue conserved in a subset of homologous enzymes is required for formation of the trimetal site and can also serve as a labile ligand to the dimetal site Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.52 90000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.52 3',5'-di-cGMP + H2O Thermotoga maritima
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 3',5'-di-cGMP + H2O Thermotoga maritima DSM 3109
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 3',5'-di-cGMP + H2O Thermotoga maritima ATCC 43589
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima
-
 2 5'-GMP
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima DSM 3109
-
 2 5'-GMP
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima ATCC 43589
-
 2 5'-GMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.52 Thermotoga maritima Q9WY30
-
-
3.1.4.52 Thermotoga maritima ATCC 43589 Q9WY30
-
-
3.1.4.52 Thermotoga maritima DSM 3109 Q9WY30
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.52 amylose resin column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.52 3',5'-di-cGMP + H2O
-
 Thermotoga maritima 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 3',5'-di-cGMP + H2O
-
 Thermotoga maritima DSM 3109 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 3',5'-di-cGMP + H2O
-
 Thermotoga maritima ATCC 43589 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima 2 5'-GMP
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima DSM 3109 2 5'-GMP
-
 ?
3.1.4.52 5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima ATCC 43589 2 5'-GMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.52 ? x * 90000, His6- and maltose binding protein-tagged enzyme, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.4.52 c-di-GMP phosphodiesterase
-
 Thermotoga maritima
3.1.4.52 cyclic di-GMP phosphodiesterase
-
 Thermotoga maritima
3.1.4.52 HD-GYP
-
 Thermotoga maritima
3.1.4.52 TM0186
-
 Thermotoga maritima
3.1.4.52 TM_0186
-
 Thermotoga maritima