EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.62 | expressed in Escherichia coli BL21(DE3) pLysS cells | Rhodococcus sp. M777 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.62 | hanging drop vapor diffusion method, using 1% (v/v) Triton X-100, 10% (v/v) glycerol, and 25% (w/v) PEG | Rhodococcus sp. M777 |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.123 | the stability of EGCII is markedly enhanced by formation of covalent complexes with cyclophellitol activity-based probes substituted with hydrophobic moieties, as evidenced by an increased melting temperature, resistance against tryptic digestion, changes in 15N-1H transverse relaxation optimized spectroscopy spectra of the [15N]Leu-labeled enzyme, and relative hydrophobicity as determined by 8-anilino-1-naphthalenesulfonic acid fluorescence. The stabilization of EGCII conformation correlates with the shape and hydrophobicity of the substituents of the activity-based probes | Rhodococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.62 | nonyl-deoxynojirimycin | - |
Rhodococcus sp. M777 | |
3.2.1.123 | additional information | beta-glucoside-configured cyclophellitol-type activity-based probes are effective mechanism-based, and irreversible inhibitors | Rhodococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.62 | GM3 ganglioside + H2O | Rhodococcus sp. M777 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.62 | Rhodococcus sp. M777 | O33853 | - |
- |
3.2.1.123 | Rhodococcus sp. | O33853 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.62 | nickel affinity column chromatography | Rhodococcus sp. M777 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.62 | 4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Rhodococcus sp. M777 | 4-methylumbelliferol + beta-D-lactopyranose | - |
? | |
3.2.1.62 | GM3 ganglioside + H2O | - |
Rhodococcus sp. M777 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.62 | monomer or dimer | x * 34000 + x * 25000, SDS-PAGE | Rhodococcus sp. M777 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.62 | EGCII | - |
Rhodococcus sp. M777 |
3.2.1.62 | endoglycoceramidase II | - |
Rhodococcus sp. M777 |
3.2.1.123 | EGCII | - |
Rhodococcus sp. |
3.2.1.123 | endoglycoceramidase II | - |
Rhodococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 37 | - |
- |
Rhodococcus sp. M777 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 25 | - |
after 1 h at 25°C in 150mM McIlvaine's buffer (pH 5.5), the enzyme remains stable with no changes in its secondary structure and its active state | Rhodococcus sp. M777 |
3.2.1.62 | 42 | - |
melting temperature | Rhodococcus sp. M777 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.62 | 5.5 | - |
in 150 mM McIlvaine's buffer | Rhodococcus sp. M777 |