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Literature summary extracted from
- Variants of phosphotriesterase for the enhanced detoxification of the chemical warfare agent VR (2015), Biochemistry, 54, 5502-5512 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Brevundimonas diminuta |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | purified recombinant enzyme mutants A80V/F132V/K185R/H254Q/H257Y/I274N/S308L and I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L, vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 50 mM HEPES, pH 8.5, and 1.0 mM CoCl2, with 0.001 ml of precipitant solution containing 100 mM sodium cacodylate, pH 5.5-7.0, 0.2 M magnesium acetate, and 15-30% PEG 8000, and equilbration against 0.5 ml of the precipitant solution, 7-21 days, 18°C, X-ray diffraction structure determination and analysis, molecular replacement using the coordinates of wild-type PTE, PDB ID 1DPM, as the search model | Brevundimonas diminuta |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | A80V/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis, the mutant combines expression-enhancing mutations (A80V/K185R/I274N) with additional changes in the active site to achieve a high kcat/Km for the hydrolysis of the SP-enantiomer of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX. Three-dimensional structure, overview | Brevundimonas diminuta |
| 3.1.8.1 | A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | F132V/H254Q/H257F | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | F132V/H254Q/H257F/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | H254Q/H257F | site-directed mutagenesis, the mutant exhibits a 100fold improvement for the hydrolysis of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, relative to the wild-type enzyme | Brevundimonas diminuta |
| 3.1.8.1 | I106C | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | I106C/F132V/H254Q/H257F | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | I106C/F132V/H254Q/H257F/S308L | site-directed mutagenesis, the mutant shows a similar catalytic efficiency for the hydrolysis of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, as mutant H254Q/H257F, but a 3fold improvement in kcat | Brevundimonas diminuta |
| 3.1.8.1 | I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | site-directed mutagenesis, the mutant has a kcat value for ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, that is enhanced by 150fold relative to wild-type PTE. Three-dimensional structure, overview | Brevundimonas diminuta |
| 3.1.8.1 | I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | I106G | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | I106G/F132V/H254Q/H257F/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | L308S | site-directed mutagenesis | Brevundimonas diminuta |
| 3.1.8.1 | additional information | computational docking study of wild-type and mutant enzymes with the toxic substrates | Brevundimonas diminuta |
| 3.1.8.1 | Y309F | site-directed mutagenesis | Brevundimonas diminuta |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 0.0017 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta |  |
| 3.1.8.1 | 0.0036 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0053 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0053 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0054 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0056 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0067 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0072 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.008 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0092 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 0.0092 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.011 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.021 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.022 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.025 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.03 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.033 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.081 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 0.13 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 0.15 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.16 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.18 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 0.18 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.18 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.19 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.19 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.2 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.23 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.23 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.24 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.31 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.35 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 0.36 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 0.41 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.62 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.65 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.71 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.73 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.76 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.8 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 0.87 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 1.2 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 1.4 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 1.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 1.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 1.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 1.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 1.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 1.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 1.7 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 2 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 2.1 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 2.8 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 3.2 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 4.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | Co2+ | activates, required | Brevundimonas diminuta | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | diethyl-paraoxon + H2O | Brevundimonas diminuta | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | Brevundimonas diminuta | i.e. VX | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | Brevundimonas diminuta | P0A434 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | recombinant wild-type and mutant enzymes from cell-free enzyme extract by precipitation of nucleic acids by protamine sulfate, ammonium sulfate fractionation, and gel filtration, followed by anion exchange chromatography | Brevundimonas diminuta |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | (S)-[2-(diethylamino)ethyl] O,O-diethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| 3.1.8.1 | (S)-[2-(diethylamino)ethyl] O,O-dimethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | dimethyl phosphate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| 3.1.8.1 | (S)-[2-(diethylamino)ethyl] O-(2-methylpropyl) methylphosphonothioate + H2O | - |
Brevundimonas diminuta | 2-methylpropyl methylphosphonate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| 3.1.8.1 | (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| 3.1.8.1 | (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-dimethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | dimethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| 3.1.8.1 | (S)-[2-[di(propan-2-yl)amino]ethyl] O-ethyl methylphosphonothioate + H2O | - |
Brevundimonas diminuta | ethyl methylphosphonate + [di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| 3.1.8.1 | 4-acetylphenyl (R)-2-methylpropyl methylphosphonate + H2O | - |
Brevundimonas diminuta | (R)-2-methylpropyl methylphosphonate + 4-acetylphenol | - |
? | |
| 3.1.8.1 | 4-acetylphenyl (S)-2-methylpropyl methylphosphonate + H2O | - |
Brevundimonas diminuta | (S)-2-methylpropyl methylphosphonate + 4-acetylphenol | - |
? | |
| 3.1.8.1 | diethyl-paraoxon + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 4-nitrophenol | - |
? | |
| 3.1.8.1 | additional information | wild-type PTE exhibits little stereoselectivity against the relatively small phosphonate center of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, but the elevated pKa of the thiol-leaving group provides a significant challenge for enzyme-catalyzed hydrolysis. Wild-type PTE has enzymatic activity for the hydrolysis of racemic VR similar to VX, but the larger isobutyl group attached to the phosphorus center results in a 25fold preference for the RP-enantiomer. Computational docking study of wild-type and mutant enzymes with the substrates | Brevundimonas diminuta | ? | - |
? | |
| 3.1.8.1 | N,N-diethyl-2-(methyl-(2-methylpropoxy)phosphoryl)sulfanylethanamine + H2O | i.e. VR | Brevundimonas diminuta | ? | - |
? | |
| 3.1.8.1 | O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | i.e. VX | Brevundimonas diminuta | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | phosphotriesterase | - |
Brevundimonas diminuta |
| 3.1.8.1 | PTE | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 30 | - |
assay at | Brevundimonas diminuta |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 1.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 3 | 6 | (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 3 | 6 | 4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 5.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 6.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 8.1 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 8.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 8.7 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 13 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 14 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 14 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 15 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 16 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 17 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 19 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 21 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 22 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 23 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 25 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 29 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 31 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 33 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 33 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 35 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 38 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 40 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 41 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 45 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 46 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 48 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 50 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 51 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 51 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 53 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 55 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 56 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 57 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 58 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 58 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 66 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 72 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 84 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 85 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 100 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 101 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 103 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 108 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 116 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 122 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 131 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 159 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 160 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 166 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 174 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 446 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 456 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 545 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 2230 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 8 | 9 | assay at | Brevundimonas diminuta |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 1.264 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 4.0625 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 4.357 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 5.375 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 5.55 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 9.3 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 11.071 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 14.571 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 17.1 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 19 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 21.053 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 21.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 21.539 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 25 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 28.75 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 30 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 30.137 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 32.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 34 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 34.375 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 38.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 42.6 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 49.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3.1.8.1 | 61 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 81.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 85 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 94.1 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 110.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 112 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 124.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 143.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 158.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 162.9 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 166.67 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 180 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 255.6 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 290 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 294.4 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 313 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 333.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 535.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 883.3 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 1920 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 4925 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 6304 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 6786 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 7736 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 8824 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 9722 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 3.1.8.1 | 9818 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 3.1.8.1 | 11806 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 12222 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 14239 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3.1.8.1 | 14500 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 16515 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 19434 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 20727 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 21239 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 3.1.8.1 | 27531 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
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Release 2023.1 (January 2023)
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