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Literature summary extracted from

  • Cotton, J.L.; Tao, J.; Balibar, C.J.
    Identification and characterization of the Staphylococcus aureus gene cluster coding for staphyloferrin A (2009), Biochemistry, 48, 1025-1035 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.57 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
6.3.2.58 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.57 Mg2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.57 ATP + N5-[(S)-citryl]-D-ornithine + citrate Staphylococcus aureus
-
AMP + diphosphate + staphyloferrin A
-
?
6.3.2.57 ATP + N5-[(S)-citryl]-D-ornithine + citrate Staphylococcus aureus RN4220
-
AMP + diphosphate + staphyloferrin A
-
?
6.3.2.58 ATP + D-ornithine + citrate Staphylococcus aureus
-
AMP + diphosphate + N5-[(S)-citryl]-D-ornithine
-
?
6.3.2.58 ATP + D-ornithine + citrate Staphylococcus aureus RN4220
-
AMP + diphosphate + N5-[(S)-citryl]-D-ornithine
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.57 Staphylococcus aureus
-
-
-
6.3.2.58 Staphylococcus aureus
-
-
-
6.3.2.58 Staphylococcus aureus RN4220
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.57 Talon metal affinity column chromatography Staphylococcus aureus
6.3.2.58 TALON metal affinity resin column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.57 ATP + N5-[(S)-citryl]-D-ornithine + citrate
-
Staphylococcus aureus AMP + diphosphate + staphyloferrin A
-
?
6.3.2.57 ATP + N5-[(S)-citryl]-D-ornithine + citrate
-
Staphylococcus aureus RN4220 AMP + diphosphate + staphyloferrin A
-
?
6.3.2.58 ATP + D-ornithine + citrate
-
Staphylococcus aureus AMP + diphosphate + N5-[(S)-citryl]-D-ornithine
-
?
6.3.2.58 ATP + D-ornithine + citrate
-
Staphylococcus aureus RN4220 AMP + diphosphate + N5-[(S)-citryl]-D-ornithine
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.57 ? x * 67200, SDS-PAGE Staphylococcus aureus
6.3.2.58 ? x * 76100, calculated from amino acid sequence Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.57 sfnaB
-
Staphylococcus aureus
6.3.2.58 sfnaD
-
Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.57 ATP
-
Staphylococcus aureus
6.3.2.58 ATP
-
Staphylococcus aureus