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Literature summary extracted from

  • French, J.B.; Cen, Y.; Sauve, A.A.
    Plasmodium falciparum Sir2 is an NAD+-dependent deacetylase and an acetyllysine-dependent and acetyllysine-independent NAD+ glycohydrolase (2008), Biochemistry, 47, 10227-10239 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.286 expressed in Escherichia coli Codon-plus RIPL cells Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.286 alpha-1-O-methyl-adenosine diphosphoribose
-
 Plasmodium falciparum
2.3.1.286 nicotinamide
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.286 0.085
-
 [peptide p300]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
2.3.1.286 0.176
-
 [histone H4]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
2.3.1.286 0.372
-
 [histone H3]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.286 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.286 Ni-column affinity chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.286 [histone H3]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence ARTKQTAR(K-Ac)STGG(K-Ac)APRKQLAS. The enzyme deacetylates K9 and K16 of diacetylated histone H3 Plasmodium falciparum [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
2.3.1.286 [histone H4]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence SGRG(K-Ac)GG(K-Ac)GLG(K-Ac)GGA(K-Ac)RHR. The enzyme predominantly deacetylates at K16 of tetra-acetylated histone H4 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?
2.3.1.286 [peptide p300]-N6-acetyl-L-lysine + NAD+ + H2O peptide p300 with the sequence ERSTEL(K-Ac)TEI(K-Ac)EEEDQPSTS. The enzyme predominantly deacetylates at K1040 of full length peptide p300 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.286 Sir2
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.286 0.00025
-
 [histone H3]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
2.3.1.286 0.00035
-
 [histone H4]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum
2.3.1.286 0.00092
-
 [peptide p300]-N6-acetyl-L-lysine at pH 8.0 and 37°C Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.286 NAD+ dependent on Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.286 0.035
-
 nicotinamide with [histone H3]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C Plasmodium falciparum
2.3.1.286 0.091
-
 nicotinamide with [peptide p300]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C Plasmodium falciparum