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Literature summary extracted from

  • Fujita, S.; Cho, S.H.; Yoshida, A.; Hasebe, F.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
    Crystal structure of LysK, an enzyme catalyzing the last step of lysine biosynthesis in Thermus thermophilus, in complex with lysine Insight into the mechanism for recognition of the amino-group carrier protein, LysW (2017), Biochem. Biophys. Res. Commun., 491, 409-415 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.130
-
 Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.130 LysK in complex with lysine at a resolution of 2.4 A. The alpha-amino group of the bound lysine is oriented toward the catalytic center, which is composed of the residues coordinating divalent metal ions for the hydrolysis of the isopeptide bond. An 11 A long path leads from the active site binding lysine to the protein surface. A positively-charged surface region is located around the exit of the path Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.130 D325A marked decrease in activity Thermus thermophilus
3.5.1.130 K297E marked decrease in activity Thermus thermophilus
3.5.1.130 R282E marked decrease in activity Thermus thermophilus
3.5.1.130 R286E marked decrease in activity Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.130 Thermus thermophilus Q8VUS5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.130 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O
-
 Thermus thermophilus [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
 ?
3.5.1.130 [CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O i.e. the C-terminal 10 residues of LysW fused with one lysine residue at its gamma-carboxyl group of C-terminal Glu54 Thermus thermophilus [CT10-LysW]-C-terminal-L-glutamate + L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.130 lysK
-
 Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.130 3.1
-
 [CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate mutant R286E, pH 8.0, 60°C Thermus thermophilus
3.5.1.130 4.5
-
 [CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate mutant R282E, pH 8.0, 60°C Thermus thermophilus
3.5.1.130 5.5
-
 [CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate wild-type, pH 8.0, 60°C Thermus thermophilus
3.5.1.130 36
-
 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate mutant R286E, pH 8.0, 60°C Thermus thermophilus
3.5.1.130 38
-
 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate mutant R282E, pH 8.0, 60°C Thermus thermophilus
3.5.1.130 530
-
 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate wild-type, pH 8.0, 60°C Thermus thermophilus