EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.40 | the recombinant protein is fused to a C-terminal His-tag and expressed in Escherichia coli BL21(DE3) | Novosphingobium sp. PP1Y |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.40 | D503A | no detectable activity | Novosphingobium sp. PP1Y |
3.2.1.40 | D552A | kcat/KM value is 65% of the wild-type value. This reduced catalytic efficiency is mainly due to a significative variation in the turnover number, defined by the kcat value, for which a 34% decrease is observed | Novosphingobium sp. PP1Y |
3.2.1.40 | D552A | mutant enzyme shows 47.9% of residual activity | Novosphingobium sp. PP1Y |
3.2.1.40 | D763A | kcat/KM value is 21% of the wild-type value. A 82% decrease in kcat is retrieved for the mutant enzyme, while the KM is similar within the experimental error | Novosphingobium sp. PP1Y |
3.2.1.40 | D763A | mutant enzyme shows 15.8% of residual activity | Novosphingobium sp. PP1Y |
3.2.1.40 | E506A | mutant enzyme shows 0.05% of residual activity | Novosphingobium sp. PP1Y |
3.2.1.40 | E644A | no detectable activity | Novosphingobium sp. PP1Y |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 0.031 | - |
rutin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.067 | - |
hesperidin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.138 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D763A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.15 | - |
quercitrin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.157 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, wild-type enzyme | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.159 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D552A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 0.59 | - |
naringin | pH 6.9, 37°C | Novosphingobium sp. PP1Y |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.40 | Ca2+ | EDTA-treated enzyme recovers its activity in the presence of divalent cations such as Mg2+ and Ca2+, but with an apparent higher efficiency when calcium is used. Almost total recovery of activity in the presence of a 3000-fold excess of Ca2+ compared to the 56% obtained with a similar excess of Mg2+. Five residues contribute to the binding of the Ca2+ ion: E593, E538, D458, S459 and E561 | Novosphingobium sp. PP1Y | |
3.2.1.40 | Mg2+ | EDTA-treated enzyme recovers its activity in the presence of divalent cations such as Mg2+ and Ca2+, but with an apparent higher efficiency when calcium is used. Almost total recovery of activity in the presence of a 3000-fold excess of Ca2+ compared to the 56% obtained with a similar excess of Mg2+ | Novosphingobium sp. PP1Y |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 12000 | - |
- |
Novosphingobium sp. PP1Y |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.40 | Novosphingobium sp. PP1Y | F6IEX3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.40 | - |
Novosphingobium sp. PP1Y |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.40 | 4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Novosphingobium sp. PP1Y | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
3.2.1.40 | hesperidin + H2O | - |
Novosphingobium sp. PP1Y | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
3.2.1.40 | additional information | the enzyme is able to hydrolyze rhamnose from natural flavonoids. It hydrolyzes both alpha-1,2 and alpha-1,6 glycosidic linkages | Novosphingobium sp. PP1Y | ? | - |
? | |
3.2.1.40 | naringin + H2O | - |
Novosphingobium sp. PP1Y | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
3.2.1.40 | quercitrin + H2O | - |
Novosphingobium sp. PP1Y | quercetin + alpha-L-rhamnose | - |
? | |
3.2.1.40 | rutin + H2O | - |
Novosphingobium sp. PP1Y | isoquercitrin + alpha-L-rhamnose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.40 | monomer | - |
Novosphingobium sp. PP1Y |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.40 | GH106 alpha-L-rhamnosidase | - |
Novosphingobium sp. PP1Y |
3.2.1.40 | RHA-P | - |
Novosphingobium sp. PP1Y |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 35 | 45 | - |
Novosphingobium sp. PP1Y |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 12.2 | - |
quercitrin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 12.7 | - |
hesperidin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 18.2 | - |
rutin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 126 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D763A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 214 | - |
naringin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 458 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D552A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 692 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, wild-type enzyme | Novosphingobium sp. PP1Y |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.40 | 6 | 7.5 | - |
Novosphingobium sp. PP1Y |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.40 | 80 | - |
quercitrin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 190 | - |
hesperidin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 360 | - |
naringin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 586 | - |
rutin | pH 6.9, 37°C | Novosphingobium sp. PP1Y | |
3.2.1.40 | 900 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D763A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 2900 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, mutant enzyme D552A | Novosphingobium sp. PP1Y | |
3.2.1.40 | 4400 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | pH 6.9, 37°C, wild-type enzyme | Novosphingobium sp. PP1Y |