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Literature summary extracted from

  • Ogawa, T.; Kimura, A.; Sakuyama, H.; Tamoi, M.; Ishikawa, T.; Shigeoka, S.
    Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis (2015), Arch. Biochem. Biophys., 575, 61-68 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression in Escherichia coli strain BL21 Star (DE3) pLysS Euglena gracilis
3.1.3.11 gene EgFBPaseI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR expression analysis, recombinant expression of His6-tagged isozyme Euglena gracilis
3.1.3.11 gene EgFBPaseII, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR expression analysis, recombinant expression of His6-tagged isozyme Euglena gracilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 additional information EgFBPaseI gene suppression by by RNAi gene silencing Euglena gracilis
3.1.3.11 additional information EgFBPaseII gene suppression by by RNAi gene silencing Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 H2O2 isozyme EgFBPaseI is inhibited by 1 mM H2O2 and recovers when incubated with DTT Euglena gracilis
3.1.3.11 additional information isozyme EgFBPaseII is resistant to H2O2 up to concentrations of 1 mM; not inhibited by concentrations of AMP up to 1 mM; not inhibited by concentrations of AMP up to 1 mM Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.165
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 0.165
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication Euglena gracilis
3.1.3.11 2.2
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 2.2
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.11 chloroplast
-
 Euglena gracilis 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 36900
-
 EgFBPaseII fused with the hexahistidine-tag, SDS-PAGE Euglena gracilis
3.1.3.11 46300
-
 EgFBPaseI fused with the hexahistidine-tag, SDS-PAGE Euglena gracilis
3.1.3.11 190000
-
 about, isozyme EgFBPaseII, gel filtration Euglena gracilis
3.1.3.11 210000
-
 about, isozyme EgFBPaseI, gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Euglena gracilis
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Euglena gracilis Z
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Euglena gracilis A0A0U5BQA3
-
-
3.1.3.11 Euglena gracilis A3QSS7
-
-
3.1.3.11 Euglena gracilis Z A0A0U5BQA3
-
-
3.1.3.11 Euglena gracilis Z A3QSS7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Euglena gracilis
3.1.3.11 recombinant His6-tagged isozyme by nickel affinity chromatography Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Euglena gracilis D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Euglena gracilis Z D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 homotetramer 4 * 36900, recombinant His6-tagged isozyme EgFBPaseII, SDS-PAGE Euglena gracilis
3.1.3.11 homotetramer 4 * 46300, recombinant His6-tagged isozyme EgFBPaseI, SDS-PAGE Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 EgFBPaseI
-
 Euglena gracilis
3.1.3.11 EgFBPaseII
-
 Euglena gracilis
3.1.3.11 FBPase
-
 Euglena gracilis
3.1.3.11 fructose-1,6-bisphosphatase
-
 Euglena gracilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 0.17
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 0.17
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication Euglena gracilis
3.1.3.11 1.08
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 1.08
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.5 9
-
 Euglena gracilis
3.1.3.11 9
-
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.11 8 9.5 pH 8.0: about 40% of maximal activity, pH 9-5: about 70% of maximal activity Euglena gracilis
3.1.3.11 8 9.5 pH 8.0: about 60% of maximal activity, pH 9.5: about 70% of maximal activity Euglena gracilis

General Information

EC Number General Information Comment Organism
3.1.3.11 evolution chloroplastic FBPase isoenzymes are widely distributed in photosynthetic organisms, i.e. bacteria, blue-green and green algae, lichens, and plants Euglena gracilis
3.1.3.11 malfunction EgFBPaseI gene suppression by gene silencing markedly decreases photosynthetic activity and inhibits cell growth Euglena gracilis
3.1.3.11 metabolism the chloroplastic fructose-1,6-bisphosphatase (FBPase) is a late-limiting enzyme in the Calvin cycle Euglena gracilis
3.1.3.11 metabolism the enzyme (EgFBPaseI) is critical for the Calvin cycle in Euglena chloroplasts in order to maintain normal cell growth under photoautotrophic conditions Euglena gracilis
3.1.3.11 physiological function fructose-1,6-bisphophatase catalyzes the breakdown of fructose 1,6-bisphosphate to fructose 6-phosphate and phosphate. Two FBPase isoenzymes are identified in photosynthetic eukaryotic cells: a cytosolic form, a key enzyme in gluconeogenesis, and a chloroplastic form, a rate-limiting enzyme involved in the Calvin cycle Euglena gracilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.11 0.49
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication Euglena gracilis
3.1.3.11 0.5
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 1
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Euglena gracilis
3.1.3.11 1.03
-
 D-fructose 1,6-bisphosphate isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication Euglena gracilis