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Literature summary extracted from

  • Rui, L.; Reardon, K.F.; Wood, T.K.
    Protein engineering of toluene ortho-monooxygenase of Burkholderia cepacia G4 for regiospecific hydroxylation of indole to form various indigoid compounds (2005), Appl. Microbiol. Biotechnol., 66, 422-429 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.243 expression in Escherichia coli Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.243 A113H mutation in subunit TomA3. Mutant enzyme produces primarily isatin from indole Burkholderia cepacia
1.14.13.243 A113I mutation in subunit TomA3. Mutant enzyme produces primarily indirubin from indole Burkholderia cepacia
1.14.13.243 A113V mutation in subunit TomA3, colony turns blue. Mutant enzyme produces primarily indigo from indole Burkholderia cepacia
1.14.13.243 V106A/A113G mutation in subunit TomA3. Mutant enzyme produces primarily isatin from indole Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.243 Burkholderia cepacia Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
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1.14.13.243 Burkholderia cepacia G4 Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.243 indole + NADH + H+ + O2
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 Burkholderia cepacia isoindigo + NAD+ + H2O
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 ?
1.14.13.243 indole + NADH + H+ + O2
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 Burkholderia cepacia G4 isoindigo + NAD+ + H2O
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 ?
1.14.13.243 additional information wild-type forms isoindigo via C-2 hydroxylation of the indole pyrrole. Mutant TomA3 A113G produces 4-hydroxyindole. TomA3 V106/A113 mutants with hydrophobic, polar, or charged amino acids hydroxylate indole at the C-3 and C-2 positions, forming isatin, indigo, and indirubin in a variety of distributions Burkholderia cepacia ?
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 ?
1.14.13.243 additional information wild-type forms isoindigo via C-2 hydroxylation of the indole pyrrole. Mutant TomA3 A113G produces 4-hydroxyindole. TomA3 V106/A113 mutants with hydrophobic, polar, or charged amino acids hydroxylate indole at the C-3 and C-2 positions, forming isatin, indigo, and indirubin in a variety of distributions Burkholderia cepacia G4 ?
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 ?