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Literature summary extracted from

  • Bojarova, P.; Kulik, N.; Slamova, K.; Hubalek, M.; Kotik, M.; Cvacka, J.; Pelantova, H.; Kren, V.
    Selective beta-N-acetylhexosaminidase from Aspergillus versicolor-a tool for producing bioactive carbohydrates (2019), Appl. Microbiol. Biotechnol., 103, 1737-1753 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Pichia pastoris strain KM71H Aspergillus versicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene
-
 Aspergillus versicolor
3.2.1.52 beta-D-GalNAc-(1->4)beta-D-GlcNAc
-
 Aspergillus versicolor
3.2.1.52 lactose
-
 Aspergillus versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.18
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
3.2.1.52 0.22
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 170000
-
 MALDI TOF mass spectrometry Aspergillus versicolor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Aspergillus versicolor A0A1L9P8I4
-
-
3.2.1.52 Aspergillus versicolor CCF 2491 A0A1L9P8I4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein
-
 Aspergillus versicolor

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 Q-Sepharose column chromatography Aspergillus versicolor

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 4°C, purified and concentrated enzyme in McIlvaine buffer pH 5.0 conserved with 0.03% (w/v) NaN3, ca 6 months, less than 20% loss of activity Aspergillus versicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Aspergillus versicolor 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Aspergillus versicolor CCF 2491 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Aspergillus versicolor 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Aspergillus versicolor CCF 2491 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 56872, catalytic domain, calculated from amino acid sequence Aspergillus versicolor
3.2.1.52 heterodimer 1 * 78000 + 1 * 64000, SDS-PAGE Aspergillus versicolor

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 Hex
-
 Aspergillus versicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 55
-
-
 Aspergillus versicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 40 60 the recombinant enzyme shows more than 80% activity between 40 and 60°C. The wild type enzyme shows about 65% activity at 45°C, 90% activity at 50°C, 100% activity at 55°C and less than 60% activity at 60°C Aspergillus versicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 50
-
 the enzyme is relatively unstable at 50°C (within 1 h its activity declines to ca 6%) Aspergillus versicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 8.13
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
3.2.1.52 35.3
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.5
-
 recombinant enzyme Aspergillus versicolor
3.2.1.52 6
-
 wild type enzyme Aspergillus versicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 7 the recombinant enzyme shows more than 70% activity between pH 3.0 and 6.0. The wild type enzyme shows more than 80% activity between pH 3.0 and 7.0 Aspergillus versicolor

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.52 0.0096
-
 recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor 1,2,3-tris-[1-(2-acetamido-2-deoxy-beta-D-galactopyranosyl-(1->4)-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1H-1,2,3-triazol-4-yl)-2-ethyloxy]benzene
3.2.1.52 0.055
-
 recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor beta-D-GalNAc-(1->4)beta-D-GlcNAc
3.2.1.52 0.153
-
 recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor lactose

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.52 45.2
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide recombinant enzyme, at pH 5.0 and 25°C Aspergillus versicolor
3.2.1.52 160.5
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide wild type enzyme, at pH 5.0 and 25°C Aspergillus versicolor