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Literature summary extracted from

  • de Araujo, E.A.; de Oliveira Neto, M.; Polikarpov, I.
    Biochemical characterization and low-resolution SAXS structure of two-domain endoglucanase BlCel9 from Bacillus licheniformis (2019), Appl. Microbiol. Biotechnol., 103, 1275-1287 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene cel9 or celA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus licheniformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 enzyme structure analysis using small-angle X-ray scattering at 30 A resolution and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+ slight inhibition Bacillus licheniformis
3.2.1.4 Co2+ moderate inhibition Bacillus licheniformis
3.2.1.4 Cu2+ strong inhibition Bacillus licheniformis
3.2.1.4 DMSO moderate inhibition Bacillus licheniformis
3.2.1.4 EDTA slight inhibition Bacillus licheniformis
3.2.1.4 Fe2+ strong inhibition Bacillus licheniformis
3.2.1.4 K+ slight inhibition Bacillus licheniformis
3.2.1.4 Li+ slight inhibition Bacillus licheniformis
3.2.1.4 Mn2+ slight inhibition Bacillus licheniformis
3.2.1.4 Ni2+ slight inhibition Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus licheniformis Q65JI9
-
-
3.2.1.4 Bacillus licheniformis ATCC 14580 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis DSM 13 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis Gibson 46 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis JCM 2505 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis NBRC 12200 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis NCIMB 9375 Q65JI9
-
-
3.2.1.4 Bacillus licheniformis NRRL NRS-1264 Q65JI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by TEV protease, again nickel affinity chromatography, and gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis NCIMB 9375 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis Gibson 46 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis JCM 2505 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis NRRL NRS-1264 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis NBRC 12200 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis ATCC 14580 ?
-
?
3.2.1.4 bacterial cellulose + H2O
-
Bacillus licheniformis DSM 13 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis NCIMB 9375 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis Gibson 46 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis JCM 2505 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis NRRL NRS-1264 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis NBRC 12200 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis ATCC 14580 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Bacillus licheniformis DSM 13 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NCIMB 9375 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis Gibson 46 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis JCM 2505 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NRRL NRS-1264 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NBRC 12200 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis ATCC 14580 ?
-
?
3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis DSM 13 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NCIMB 9375 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis Gibson 46 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis JCM 2505 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NRRL NRS-1264 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NBRC 12200 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis ATCC 14580 ?
-
?
3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis DSM 13 ?
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NCIMB 9375 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis Gibson 46 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis JCM 2505 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NRRL NRS-1264 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NBRC 12200 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis ATCC 14580 D-glucose + cellobiose + cellotriose + cellotetraose
-
?
3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis DSM 13 D-glucose + cellobiose + cellotriose + cellotetraose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 BlCel9
-
Bacillus licheniformis
3.2.1.4 endoglucanase
-
Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 35 75 over 50% of maximal activity within this range Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
purified recombinant enzyme, completely stable to slightly activated after 48 h Bacillus licheniformis
3.2.1.4 50
-
purified recombinant enzyme, 80% activity remaining after 48 h Bacillus licheniformis
3.2.1.4 60
-
purified recombinant enzyme, 50% activity remaining after 3 h, 20% after 6 h, and inactivation after 12 h Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 7
-
-
Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 8.2 over 50% of maximal activity within this range Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4
-
purified recombinant enzyme, Tm is 35°C Bacillus licheniformis
3.2.1.4 5 9 purified recombinant enzyme, Tm is about 75°C within this range Bacillus licheniformis
3.2.1.4 10
-
purified recombinant enzyme, Tm is 55°C Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.2.1.4 additional information enzyme structure analysis using small-angle X-ray scattering and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons Bacillus licheniformis