EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.4 | gene cel9 or celA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus licheniformis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.4 | enzyme structure analysis using small-angle X-ray scattering at 30 A resolution and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons | Bacillus licheniformis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | Ca2+ | slight inhibition | Bacillus licheniformis | |
3.2.1.4 | Co2+ | moderate inhibition | Bacillus licheniformis | |
3.2.1.4 | Cu2+ | strong inhibition | Bacillus licheniformis | |
3.2.1.4 | DMSO | moderate inhibition | Bacillus licheniformis | |
3.2.1.4 | EDTA | slight inhibition | Bacillus licheniformis | |
3.2.1.4 | Fe2+ | strong inhibition | Bacillus licheniformis | |
3.2.1.4 | K+ | slight inhibition | Bacillus licheniformis | |
3.2.1.4 | Li+ | slight inhibition | Bacillus licheniformis | |
3.2.1.4 | Mn2+ | slight inhibition | Bacillus licheniformis | |
3.2.1.4 | Ni2+ | slight inhibition | Bacillus licheniformis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Bacillus licheniformis | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis ATCC 14580 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis DSM 13 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis Gibson 46 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis JCM 2505 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis NBRC 12200 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis NCIMB 9375 | Q65JI9 | - |
- |
3.2.1.4 | Bacillus licheniformis NRRL NRS-1264 | Q65JI9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.4 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by TEV protease, again nickel affinity chromatography, and gel filtration | Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis Gibson 46 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis JCM 2505 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis NBRC 12200 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis ATCC 14580 | ? | - |
? | |
3.2.1.4 | bacterial cellulose + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis Gibson 46 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis JCM 2505 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis NBRC 12200 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis ATCC 14580 | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis Gibson 46 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis JCM 2505 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis NBRC 12200 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
3.2.1.4 | filter paper + H2O | qualitative filter paper-grade 1 | Bacillus licheniformis DSM 13 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NCIMB 9375 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis Gibson 46 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis JCM 2505 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NRRL NRS-1264 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis NBRC 12200 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis ATCC 14580 | ? | - |
? | |
3.2.1.4 | additional information | substrate specificity, overview. Poor activity with Avicel PH-101 | Bacillus licheniformis DSM 13 | ? | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NCIMB 9375 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis Gibson 46 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis JCM 2505 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NRRL NRS-1264 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis NBRC 12200 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis ATCC 14580 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? | |
3.2.1.4 | phosphoric acid swollen cellulose + H2O | best substrate | Bacillus licheniformis DSM 13 | D-glucose + cellobiose + cellotriose + cellotetraose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | BlCel9 | - |
Bacillus licheniformis |
3.2.1.4 | endoglucanase | - |
Bacillus licheniformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 60 | - |
- |
Bacillus licheniformis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 35 | 75 | over 50% of maximal activity within this range | Bacillus licheniformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 40 | - |
purified recombinant enzyme, completely stable to slightly activated after 48 h | Bacillus licheniformis |
3.2.1.4 | 50 | - |
purified recombinant enzyme, 80% activity remaining after 48 h | Bacillus licheniformis |
3.2.1.4 | 60 | - |
purified recombinant enzyme, 50% activity remaining after 3 h, 20% after 6 h, and inactivation after 12 h | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 7 | - |
- |
Bacillus licheniformis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 4 | 8.2 | over 50% of maximal activity within this range | Bacillus licheniformis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 4 | - |
purified recombinant enzyme, Tm is 35°C | Bacillus licheniformis |
3.2.1.4 | 5 | 9 | purified recombinant enzyme, Tm is about 75°C within this range | Bacillus licheniformis |
3.2.1.4 | 10 | - |
purified recombinant enzyme, Tm is 55°C | Bacillus licheniformis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.4 | additional information | enzyme structure analysis using small-angle X-ray scattering and homology modelling with the crystallographic model of the endoglucanase TfCel9A (PDB ID 1JS4), structure comparisons | Bacillus licheniformis |