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Literature summary extracted from

  • Ontanon, O.M.; Ghio, S.; Marrero Diaz de Villegas, R.; Piccinni, F.E.; Talia, P.M.; Cerutti, M.L.; Campos, E.
    EcXyl43 beta-xylosidase molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction (2018), Appl. Microbiol. Biotechnol., 102, 6959-6971 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 additional information the enzyme can be a suitable additive for hemicellulases in long-term hydrolysis reactions. Because of its moderate inhibition by monomeric sugars but its high inhibition by ethanol, the enzyme can be particularly more useful in separate hydrolysis and fermentation than in simultaneous saccharification and co-fermentation or consolidated bioprocessing Enterobacter sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Escherichia coli BL21 Enterobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 alpha-L-arabinofuranose competitive Enterobacter sp.
3.2.1.37 beta-D-xylose competitive Enterobacter sp.
3.2.1.37 D-glucose non-competitive Enterobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 9.9
-
 xylohexaose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 10.3
-
 4-nitrophenyl beta-D-xylopyranoside 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 12.3
-
 xylopentaose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 18.4
-
 xylotetraose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 18.6
-
 xylotriose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 21.2
-
 xylobiose 40°C, pH 6.5 Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Enterobacter sp. V5J3M5 enrichment culture clone nf1B6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Enterobacter sp. 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Enterobacter sp. 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 xylan + H2O low levels of activity on commercial xylan were also observed, mainly on the soluble fraction Enterobacter sp. beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Enterobacter sp. 2 beta-D-xylose
-
 ?
3.2.1.37 xylohexaose + H2O
-
 Enterobacter sp. beta-D-xylose + ?
-
 ?
3.2.1.37 xylooligosaccharide + H2O the enzyme hydrolyzes xylooligosaccharides, up to degree of polymerization 6, with higher activity towards longer xylooligosaccharide Enterobacter sp. beta-D-xylose + ?
-
 ?
3.2.1.37 xylopentaose + H2O
-
 Enterobacter sp. beta-D-xylose + ?
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Enterobacter sp. beta-D-xylose + ?
-
 ?
3.2.1.37 xylotriose + H2O
-
 Enterobacter sp. beta-D-xylose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 EcXyl43 beta-xylosidase
-
 Enterobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 40
-
-
 Enterobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 40
-
 t1/2: 58.73h Enterobacter sp.
3.2.1.37 45
-
 t1/2: 5.71 h Enterobacter sp.
3.2.1.37 50
-
 t1/2: 0.14 h, without Ca2+, the addition of Ca2+ improves enzyme stability at 50 °C Enterobacter sp.
3.2.1.37 50
-
 t1/2: 0.51 h, 10 mM Ca2+ Enterobacter sp.
3.2.1.37 50
-
 t1/2: 0.6 h, 5 mM Ca2+ Enterobacter sp.
3.2.1.37 50
-
 t1/2: 0.64 h, 1 mM Ca2+ Enterobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 1.9
-
 xylobiose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 3
-
 xylotriose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 5.6
-
 xylohexaose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 5.8
-
 xylopentaose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 6
-
 xylotetraose 40°C, pH 6.5 Enterobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 6.5
-
-
 Enterobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.37 79
-
 beta-D-xylose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 102
-
 alpha-L-arabinofuranose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 350
-
 D-glucose 40°C, pH 6.5 Enterobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 0.09
-
 xylobiose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 0.16
-
 xylotriose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 0.33
-
 xylotetraose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 0.47
-
 xylopentaose 40°C, pH 6.5 Enterobacter sp.
3.2.1.37 0.57
-
 xylohexaose 40°C, pH 6.5 Enterobacter sp.