BRENDA - Enzyme Database

EcXyl43 beta-xylosidase molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction

Ontanon, O.M.; Ghio, S.; Marrero Diaz de Villegas, R.; Piccinni, F.E.; Talia, P.M.; Cerutti, M.L.; Campos, E.; Appl. Microbiol. Biotechnol. 102, 6959-6971 (2018) View publication on PubMed

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.2.1.37
additional information
the enzyme can be a suitable additive for hemicellulases in long-term hydrolysis reactions. Because of its moderate inhibition by monomeric sugars but its high inhibition by ethanol, the enzyme can be particularly more useful in separate hydrolysis and fermentation than in simultaneous saccharification and co-fermentation or consolidated bioprocessing
Enterobacter sp.
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.37
expression in Escherichia coli BL21
Enterobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
alpha-L-arabinofuranose
competitive
Enterobacter sp.
3.2.1.37
beta-D-xylose
competitive
Enterobacter sp.
3.2.1.37
D-glucose
non-competitive
Enterobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.37
9.9
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
10.3
-
4-nitrophenyl beta-D-xylopyranoside
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
12.3
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
18.4
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
18.6
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
21.2
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.37
Enterobacter sp.
V5J3M5
enrichment culture clone nf1B6
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.37
-
Enterobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.37
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
749706
Enterobacter sp.
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
3.2.1.37
4-nitrophenyl beta-D-xylopyranoside + H2O
-
749706
Enterobacter sp.
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
3.2.1.37
xylan + H2O
low levels of activity on commercial xylan were also observed, mainly on the soluble fraction
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylobiose + H2O
-
749706
Enterobacter sp.
2 beta-D-xylose
-
-
-
?
3.2.1.37
xylohexaose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylooligosaccharide + H2O
the enzyme hydrolyzes xylooligosaccharides, up to degree of polymerization 6, with higher activity towards longer xylooligosaccharide
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylopentaose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylotetraose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylotriose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.37
EcXyl43 beta-xylosidase
-
Enterobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
-
Enterobacter sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
t1/2: 58.73h
Enterobacter sp.
3.2.1.37
45
-
t1/2: 5.71 h
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.14 h, without Ca2+, the addition of Ca2+ improves enzyme stability at 50 °C
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.51 h, 10 mM Ca2+
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.6 h, 5 mM Ca2+
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.64 h, 1 mM Ca2+
Enterobacter sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.37
1.9
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
3
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
5.6
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
5.8
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
6
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
6.5
-
-
Enterobacter sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.37
79
-
beta-D-xylose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
102
-
alpha-L-arabinofuranose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
350
-
D-glucose
40°C, pH 6.5
Enterobacter sp.
Application (protein specific)
EC Number
Application
Commentary
Organism
3.2.1.37
additional information
the enzyme can be a suitable additive for hemicellulases in long-term hydrolysis reactions. Because of its moderate inhibition by monomeric sugars but its high inhibition by ethanol, the enzyme can be particularly more useful in separate hydrolysis and fermentation than in simultaneous saccharification and co-fermentation or consolidated bioprocessing
Enterobacter sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
expression in Escherichia coli BL21
Enterobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
alpha-L-arabinofuranose
competitive
Enterobacter sp.
3.2.1.37
beta-D-xylose
competitive
Enterobacter sp.
3.2.1.37
D-glucose
non-competitive
Enterobacter sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.37
79
-
beta-D-xylose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
102
-
alpha-L-arabinofuranose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
350
-
D-glucose
40°C, pH 6.5
Enterobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.37
9.9
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
10.3
-
4-nitrophenyl beta-D-xylopyranoside
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
12.3
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
18.4
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
18.6
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
21.2
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
-
Enterobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.37
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
749706
Enterobacter sp.
4-nitrophenol + alpha-L-arabinofuranose
-
-
-
?
3.2.1.37
4-nitrophenyl beta-D-xylopyranoside + H2O
-
749706
Enterobacter sp.
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
3.2.1.37
xylan + H2O
low levels of activity on commercial xylan were also observed, mainly on the soluble fraction
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylobiose + H2O
-
749706
Enterobacter sp.
2 beta-D-xylose
-
-
-
?
3.2.1.37
xylohexaose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylooligosaccharide + H2O
the enzyme hydrolyzes xylooligosaccharides, up to degree of polymerization 6, with higher activity towards longer xylooligosaccharide
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylopentaose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylotetraose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
3.2.1.37
xylotriose + H2O
-
749706
Enterobacter sp.
beta-D-xylose + ?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
-
Enterobacter sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
t1/2: 58.73h
Enterobacter sp.
3.2.1.37
45
-
t1/2: 5.71 h
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.14 h, without Ca2+, the addition of Ca2+ improves enzyme stability at 50 °C
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.51 h, 10 mM Ca2+
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.6 h, 5 mM Ca2+
Enterobacter sp.
3.2.1.37
50
-
t1/2: 0.64 h, 1 mM Ca2+
Enterobacter sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.37
1.9
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
3
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
5.6
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
5.8
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
6
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
6.5
-
-
Enterobacter sp.
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.37
0.09
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.16
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.33
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.47
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.57
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.37
0.09
-
xylobiose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.16
-
xylotriose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.33
-
xylotetraose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.47
-
xylopentaose
40°C, pH 6.5
Enterobacter sp.
3.2.1.37
0.57
-
xylohexaose
40°C, pH 6.5
Enterobacter sp.