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Literature summary extracted from

  • Zhang, W.; Xu, J.; Liu, D.; Liu, H.; Lu, X.; Yu, W.
    Characterization of an alpha-agarase from Thalassomonas sp. LD5 and its hydrolysate (2018), Appl. Microbiol. Biotechnol., 102, 2203-2212 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.158 expressed in Escherichia coli AD494(DE3) cells Thalassomonas sp. LD5

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.158 Al3+ 3% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Ba2+ 90% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Cu2+ 5% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 EDTA 1% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Fe2+ 18% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Fe3+ complete inhibition at 2 mM Thalassomonas sp. LD5
3.2.1.158 Mg2+ 70% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Mn2+ complete inhibition at 2 mM Thalassomonas sp. LD5
3.2.1.158 NH4+ 84% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Ni2+ 28% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 SDS 39% residual activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Zn2+ 10% residual activity at 2 mM Thalassomonas sp. LD5

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.158 Ca2+ the enzyme is strongly dependent on the presence of Ca2+ ions (170% activity at 10 mM) Thalassomonas sp. LD5
3.2.1.158 Li+ 105% activity at 2 mM Thalassomonas sp. LD5
3.2.1.158 Na+ 107% activity at 2 mM Thalassomonas sp. LD5

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.158 360000
-
 gel filtration Thalassomonas sp. LD5

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.158 Thalassomonas sp. LD5 A0A1S5VH75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.158 Ni-Sepharose column chromatography and Superdex S200 gel filtration Thalassomonas sp. LD5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.158 agar + H2O
-
 Thalassomonas sp. LD5 agarotetraose
-
 ?
3.2.1.158 agarotetraose + H2O
-
 Thalassomonas sp. LD5 neoagarotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.158 homodimer 2 * 180000, SDS-PAGE Thalassomonas sp. LD5
3.2.1.158 homodimer 2 * 154000, calculated from amino acid sequence Thalassomonas sp. LD5

Synonyms

EC Number Synonyms Comment Organism
3.2.1.158 AgaD
-
 Thalassomonas sp. LD5

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.158 35
-
-
 Thalassomonas sp. LD5

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.158 30 45 more than 50% of the enzyme activity is lost below 30°C and above 45°C Thalassomonas sp. LD5

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.158 10 40 the enzyme retains 100% or 60% activity after being incubated at 10-20°C or 30°C for 1 h, respectively. The enzyme loses nearly 95% activity after being incubated at 40°C for 1 h Thalassomonas sp. LD5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.158 8.5
-
-
 Thalassomonas sp. LD5