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Literature summary extracted from

  • Alvin, A.; Kim, J.; Jeong, G.T.; Tsang, Y.F.; Kwon, E.E.; Neilan, B.A.; Jeon, Y.J.
    Industrial robustness linked to the gluconolactonase from Zymomonas mobilis (2017), Appl. Microbiol. Biotechnol., 101, 5089-5099 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.17 additional information the potential gluconolactone production of the gluconolactonase-deficient strain Zymomonas mobilis ZM4 gnlDELTA, which has completely lost its gluconolactonase activity, are investigated via growth inhibitory assay and biotransformation of glucose and fructose into gluconolactone and sorbitol, respectively. The mutant strain shows severe growth inhibitions in the presence of 2 mM hydrogen peroxide, indicating that it is more susceptible to oxidative stress conditions than the wild-type strain Zymomonas mobilis subsp. mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.17 D-glucono-1,5-lactone + H2O Zymomonas mobilis subsp. mobilis
-
 D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O Zymomonas mobilis subsp. mobilis ZM4
-
 D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O Zymomonas mobilis subsp. mobilis CP4
-
 D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O Zymomonas mobilis subsp. mobilis ATCC 31821
-
 D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.17 Zymomonas mobilis subsp. mobilis Q01578
-
-
3.1.1.17 Zymomonas mobilis subsp. mobilis ATCC 31821 Q01578
-
-
3.1.1.17 Zymomonas mobilis subsp. mobilis CP4 Q01578
-
-
3.1.1.17 Zymomonas mobilis subsp. mobilis ZM4 Q01578
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.17 1894
-
 wild-type strain, pH 6.8, 25°C Zymomonas mobilis subsp. mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Zymomonas mobilis subsp. mobilis D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Zymomonas mobilis subsp. mobilis ZM4 D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Zymomonas mobilis subsp. mobilis CP4 D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Zymomonas mobilis subsp. mobilis ATCC 31821 D-gluconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.17 GNL
-
 Zymomonas mobilis subsp. mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.17 25
-
 assay at Zymomonas mobilis subsp. mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.17 6.8
-
 assay at Zymomonas mobilis subsp. mobilis