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Literature summary extracted from

  • Honda, S.; Wakita, S.; Sugahara, Y.; Kawakita, M.; Oyama, F.; Sakaguchi, M.
    Characterization of two Listeria innocua chitinases of different sizes that were expressed in Escherichia coli (2016), Appl. Microbiol. Biotechnol., 100, 8031-8041 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Escherichia coli BL21(DE3) cells Listeria innocua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.127
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi78, at pH 6.0 and 50°C Listeria innocua
3.2.1.14 0.885
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi35, at pH 5.0 and 50°C Listeria innocua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Listeria innocua
-
 ?
-
 ?
3.2.1.14 chitin + H2O Listeria innocua ATCC 33090
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Listeria innocua A0A146FAY1
-
-
3.2.1.14 Listeria innocua A0A146FAZ1
-
-
3.2.1.14 Listeria innocua ATCC 33090 A0A146FAY1
-
-
3.2.1.14 Listeria innocua ATCC 33090 A0A146FAZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 Ni Sepharose column chromatography Listeria innocua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Listeria innocua 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Listeria innocua ATCC 33090 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Listeria innocua 4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Listeria innocua ATCC 33090 4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
 ?
3.2.1.14 beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
-
 Listeria innocua beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
-
 Listeria innocua ATCC 33090 beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
-
 Listeria innocua beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
 ?
3.2.1.14 beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
-
 Listeria innocua ATCC 33090 beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
 ?
3.2.1.14 chitin + H2O
-
 Listeria innocua ?
-
 ?
3.2.1.14 chitin + H2O
-
 Listeria innocua ATCC 33090 ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Listeria innocua beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Listeria innocua ATCC 33090 beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Listeria innocua N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Listeria innocua ATCC 33090 N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 additional information isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose Listeria innocua ?
-
 ?
3.2.1.14 additional information isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose Listeria innocua ATCC 33090 ?
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
-
 Listeria innocua N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
-
 Listeria innocua ATCC 33090 N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
-
 Listeria innocua N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose
-
 ?
3.2.1.14 tri-N-acetyl-chitotriose + H2O substrate for isoform Chi78 Listeria innocua beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 38000, isoform Chi35, SDS-PAGE Listeria innocua
3.2.1.14 ? x * 82000, isoform Chi78, SDS-PAGE Listeria innocua

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi35 isoform Listeria innocua
3.2.1.14 Chi78 isoform Listeria innocua

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Listeria innocua

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40 60 the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi35 has a melting temperature of 60.8°C Listeria innocua
3.2.1.14 40 60 the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi78 is almost completely inactivated in 60 min at 60°C and shows a melting temperature of 51.5°C Listeria innocua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 10.4
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi78, at pH 6.0 and 50°C Listeria innocua
3.2.1.14 51.1
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi35, at pH 5.0 and 50°C Listeria innocua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Listeria innocua
3.2.1.14 6
-
-
 Listeria innocua

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3.5
-
 isoform Chi35 retains full activity at pH 3.5 for 24 h Listeria innocua
3.2.1.14 3.5
-
 the activity of isoform Chi78 is decreased to approximately 20% in 24 h at pH 3.5 at 4 °C Listeria innocua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 60
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi35, at pH 5.0 and 50°C Listeria innocua
3.2.1.14 80
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside isoform Chi78, at pH 6.0 and 50°C Listeria innocua