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Literature summary extracted from

  • Ziesack, M.; Rollins, N.; Shah, A.; Dusel, B.; Webster, G.; Silver, P.A.; Way, J.C.
    Chimeric fatty acyl-acyl carrier protein thioesterases provide mechanistic insight into enzyme specificity and expression (2018), Appl. Environ. Microbiol., 84, e02868-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expressed in Escherichia coli BL21(DE3) cells Cuphea palustris

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.14 E235Q the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
3.1.2.14 L242F the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
3.1.2.14 M237V the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
3.1.2.14 S225N the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
3.1.2.14 S249G the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris
3.1.2.14 S269A the mutation leads to enhanced fatty acid production compared to the wild type Cuphea palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O Cuphea palustris
-
 an [acyl-carrier protein] + oleate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Cuphea palustris Q39554 and Q39555 Q39554 for isoform FatB1 and Q39555 for isoform FatB2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O
-
 Cuphea palustris an [acyl-carrier protein] + oleate
-
 ?
3.1.2.14 myristoyl-[acyl-carrier protein] + H2O high specificity substrate of isoform FatB2 Cuphea palustris [acyl-carrier protein] + myristate
-
 ?
3.1.2.14 octanoyl-[acyl-carrier protein] + H2O high specificity substrate of isoform FatB1 Cuphea palustris [acyl-carrier protein] + octanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.14 homodimer
-
 Cuphea palustris

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 FATB1 isoform, specific for C8 Cuphea palustris
3.1.2.14 FatB2 isoform, specific for C14 Cuphea palustris