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Literature summary extracted from

  • Zhou, J.; Li, Z.; Zhang, H.; Wu, J.; Ye, X.; Dong, W.; Jiang, M.; Huang, Y.; Cui, Z.
    Novel maltogenic amylase CoMA from Corallococcus sp. strain EGB catalyzes the conversion of maltooligosaccharides and soluble starch to maltose (2018), Appl. Environ. Microbiol., 84, e00152-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli Corallococcus sp. EGB

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 acarbose
-
 Corallococcus sp. EGB

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 amylopectin Km-value: 10.3 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB
3.2.1.1 additional information
-
 starch Km-value: 2.9 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 59946
-
 calculated from sequence Corallococcus sp. EGB
3.2.1.1 60000
-
 SDS-PAGE Corallococcus sp. EGB

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Corallococcus sp. EGB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Corallococcus sp. EGB

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 121.3
-
 substrate: amylopectin, pH 7.0, 50°C Corallococcus sp. EGB
3.2.1.1 980
-
 substrate: starch, pH 7.0, 50°C Corallococcus sp. EGB

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 gamma-cyclodextrin + H2O
-
 Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 maltoheptaose + H2O
-
 Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 maltohexaose + H2O
-
 Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 maltotetraose + H2O
-
 Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 maltotriose + H2O no activity against isomaltotriose (alpha-glucosyl-(1->6)-alpha-glucosyl-(1->6)-glucose) Corallococcus sp. EGB maltose + ?
-
 ?
3.2.1.1 additional information the enzyme hydrolyses alpha-1,4-glycosidic bonds and not alpha-1,6-linkages. It has no activity toward maltose, alpha-cyclodextrin, beta-cyclodextrin, panose, acarbose and dextran Corallococcus sp. EGB ?
-
 ?
3.2.1.1 starch + H2O action mode is endolytic, hydrolysis products have an alpha-anomeric configuration. Degradation of 1% (w/v) starch results in the formation of oligosaccharides in the maltose to maltopentaose range at early time points, followed by an accumulation of maltose, maltotetraose, and small amounts of glucose after 1 h, and its final end products consisted of a high level of 58% (w/w) maltose without concomitant production of glucose Corallococcus sp. EGB alpha-maltose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 ComA
-
 Corallococcus sp. EGB
3.2.1.1 maltogenic amylase
-
 Corallococcus sp. EGB

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 50
-
-
 Corallococcus sp. EGB

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 25 60 25°C: about 45% of maximal activity, 60°C: about 45% of maximal activity Corallococcus sp. EGB

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 40
-
 almost all of the enzyme activity remains after the enzyme is incubated for 60 min at temperatures of below 40°C Corallococcus sp. EGB
3.2.1.1 50
-
 30 min, not stable above 50°C Corallococcus sp. EGB

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 2274.8
-
 amylopectin pH 7.0, 50°C Corallococcus sp. EGB
3.2.1.1 3021.2
-
 starch pH 7.0, 50°C Corallococcus sp. EGB

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7
-
-
 Corallococcus sp. EGB

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 6 9 pH 6.0: about 80% of maximal activity, pH 9.0: about 45% of maximal activity Corallococcus sp. EGB

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5 9 4°C, 24 h, enzyme retains more than 50% activity Corallococcus sp. EGB

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.1 Corallococcus sp. EGB calculated from sequence
-
 6.08

General Information

EC Number General Information Comment Organism
3.2.1.1 additional information the enzyme may be involved in the accumulation of maltose in Corallococcus media Corallococcus sp. EGB

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.1 220
-
 amylopectin pH 7.0, 50°C Corallococcus sp. EGB
3.2.1.1 1019.6
-
 starch pH 7.0, 50°C Corallococcus sp. EGB