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Literature summary extracted from

  • Tang, K.; Su, Y.; Brackman, G.; Cui, F.; Zhang, Y.; Shi, X.; Coenye, T.; Zhang, X.
    MomL, a novel marine-derived N-Acyl homoserine lactonase from Muricauda olearia (2015), Appl. Environ. Microbiol., 81, 774-782 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.81 drug development MomL significantly attenuates the virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans infection model, which suggests that MomL has the potential to be used as a therapeutic agent Muricauda olearia
3.1.1.81 pharmacology MomL significantly attenuates the virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans infection model, which suggests that MomL has the potential to be used as a therapeutic agent Muricauda olearia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 Escherichia coli BL21(DE3) Muricauda olearia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.81 Cu2+ 0.1 mM, complete inhibition Muricauda olearia
3.1.1.81 EDTA
-
 Muricauda olearia
3.1.1.81 Fe2+ 0.1 mM, about 80% inhibition Muricauda olearia
3.1.1.81 Ni2+ 0.1 mM, about 40% inhibition Muricauda olearia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 0.44
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 0.44
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 0.49
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 0.79
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 0.85
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 0.95
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.81 extracellular
-
 Muricauda olearia
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Ca2+ 0.1 mM, 1.4fold activation Muricauda olearia
3.1.1.81 Co2+ 0.1 mM, 1.5fold activation Muricauda olearia
3.1.1.81 Mg2+ 0.1 mM, 1.5fold activation. The purified MomL contains 0.57 equivalents of magnesium when the medium is not supplemented with extra metal ions. When the growth medium is supplemented with ZnCl2 (0.2 mM), the molar ratios of purified MomL to zinc and magnesium are determined to be 1.67 and 0.23 equivalents Muricauda olearia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 31000
-
 purified recombinant enzyme Muricauda olearia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Muricauda olearia A0A0A1EIU0
-
-
3.1.1.81 Muricauda olearia Th120 A0A0A1EIU0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Muricauda olearia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxohexanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxooctanoyl-L-homoserine
-
 ?
3.1.1.81 N-butyryl-L-homoserine lactone + H2O
-
 Muricauda olearia N-butyryl-L-homoserine
-
 ?
3.1.1.81 N-dodecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-dodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-hexanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-octanoyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL lactonase
-
 Muricauda olearia
3.1.1.81 MomL
-
 Muricauda olearia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 40
-
-
 Muricauda olearia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.81 10 60 10°C: about 30% of maximal activity, 20°C: about 65% of maximal activity, 50°C: about 90% of maximal activity, 60°C: about 15% of maximal activity Muricauda olearia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 40
-
 30 min, no loss of activity Muricauda olearia
3.1.1.81 60
-
 30 min, the enzyme retains about 30% of its activity Muricauda olearia
3.1.1.81 100
-
 30 min, the enzyme retains about 10% of its activity Muricauda olearia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 135
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 158
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 218
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 224
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 226
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 293
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.81 3
-
 3 h, about 80% loss of activity Muricauda olearia
3.1.1.81 5
-
 3 h, 75% loss of activity Muricauda olearia
3.1.1.81 6
-
 3 h, 65% loss of activity Muricauda olearia
3.1.1.81 7
-
 3 h, 10% loss of activity Muricauda olearia
3.1.1.81 8
-
 3 h, high stability Muricauda olearia
3.1.1.81 9 11 3 h, 10% loss of activity Muricauda olearia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 160
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 290
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 310
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 360
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 450
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
3.1.1.81 510
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia