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Literature summary extracted from

  • Nieto-Dominguez, M.; de Eugenio, L.I.; Barriuso, J.; Prieto, A.; Fernandez de Toro, B.; Canales-Mayordomo, A.; Martinez, M.J.
    Novel pH-stable glycoside hydrolase family 3 beta-xylosidase from Talaromyces amestolkiae an enzyme displaying regioselective transxylosylation (2015), Appl. Environ. Microbiol., 81, 6380-6392 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expressed in Escherichia coli DH5alpha cells Talaromyces amestolkiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ about 50% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 Cu2+ about 75% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 D-xylose product inhibition Talaromyces amestolkiae
3.2.1.37 Hg2+ about 10% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 additional information absence of inhibition in the presence of EDTA, dithiothreitol, and 2-mercaptoethanol Talaromyces amestolkiae
3.2.1.37 Ni2+ about 75% residual activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 Pb2+ about 90% residual activity at 5 mM in citrate buffer Talaromyces amestolkiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.17
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 0.2
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 0.2
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 0.22
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 0.22
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 0.48
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 3.6
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 5.8
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 Al3+ about 120% activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 Co2+ about 110% activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 Fe2+ about 125% activity at 5 mM in acetate buffer Talaromyces amestolkiae
3.2.1.37 Mg2+ about 110% activity at 5 mM in acetate buffer Talaromyces amestolkiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 200000
-
gel filtration Talaromyces amestolkiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Talaromyces amestolkiae A0A0H3W535
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein
-
Talaromyces amestolkiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 HiTrap column chromatography, Mono S column chromatography, and Superose 12 gel filtration Talaromyces amestolkiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 mycelium
-
Talaromyces amestolkiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + L-arabinofuranose
-
?
3.2.1.37 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + L-arabinopyranose
-
?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
Talaromyces amestolkiae 4-nitrophenol + D-xylopyranose
-
?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + xylobiose
-
Talaromyces amestolkiae 4-nitrophenol + xylotriose
-
?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + xylotriose
-
Talaromyces amestolkiae 4-nitrophenol + xylotetraose
-
?
3.2.1.37 beechwood xylan + H2O
-
Talaromyces amestolkiae D-xylose + ?
-
?
3.2.1.37 additional information no activity with 4-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-fucopyranoside Talaromyces amestolkiae ?
-
?
3.2.1.37 xylan + H2O
-
Talaromyces amestolkiae ?
-
?
3.2.1.37 xylobiose + H2O
-
Talaromyces amestolkiae 2 D-xylose
-
?
3.2.1.37 xylohexaose + H2O
-
Talaromyces amestolkiae 6 D-xylose
-
?
3.2.1.37 xylopentaose + H2O
-
Talaromyces amestolkiae 5 D-xylose
-
?
3.2.1.37 xylotetraose + 3 H2O
-
Talaromyces amestolkiae 4 D-xylose
-
?
3.2.1.37 xylotriose + H2O
-
Talaromyces amestolkiae 3 D-xylose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 homodimer 2 * 100000, SDS-PAGE Talaromyces amestolkiae
3.2.1.37 homodimer 2 * 102275, MALDI-TOF mass spectrometry Talaromyces amestolkiae
3.2.1.37 homodimer 2 * 84374, calculated from amino acid sequence Talaromyces amestolkiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-xylosidase
-
Talaromyces amestolkiae
3.2.1.37 BxTW1
-
Talaromyces amestolkiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 70
-
-
Talaromyces amestolkiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 50 70 more than 50% activity between 50 and 70°C Talaromyces amestolkiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 30 50 the enzyme retains more than 70% activity and more than 50% activity after 72 h at 30-40 or 50°C, respectively Talaromyces amestolkiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 32
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 39.2
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 51.2
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 66.1
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 143
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 173
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 183
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 220
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 229
-
beechwood xylan at pH 3.0 and 50°C Talaromyces amestolkiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 3
-
-
Talaromyces amestolkiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 2.2 5.5 about 95% activity at pH 2.2, 100% activity at pH 3.0, about 90% activity at pH 4.0, about 55% activity at pH 5.5, about 42% activity at pH 6.0, about 12% activity at pH 7.0 Talaromyces amestolkiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 2.2 9 the enzyme retains more than 80% activity after 72 h at pH 2.2-9.0 Talaromyces amestolkiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.37 1.7
-
D-xylose pH and temperature not specified in the publication Talaromyces amestolkiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.37 Talaromyces amestolkiae isoelectric focusing
-
7.6

Expression

EC Number Organism Comment Expression
3.2.1.37 Talaromyces amestolkiae the expression of the enzyme can be induced by 1% (w/v) Avicel, 1% (w/v) D-xylose, or 1-3% (w/v) beechwood xylan up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 25
-
4-nitrophenyl alpha-L-arabinofuranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 62
-
4-nitrophenyl alpha-L-arabinopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 140
-
xylohexaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 200
-
xylopentaose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 260
-
xylotetraose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 290
-
xylotriose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 380
-
xylobiose at pH 3.0 and 50°C Talaromyces amestolkiae
3.2.1.37 1000
-
4-nitrophenyl beta-D-xylopyranoside at pH 3.0 and 50°C Talaromyces amestolkiae