EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.37 | expressed in Escherichia coli DH5alpha cells | Talaromyces amestolkiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.37 | Ag+ | about 50% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | Cu2+ | about 75% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | D-xylose | product inhibition | Talaromyces amestolkiae | |
3.2.1.37 | Hg2+ | about 10% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | additional information | absence of inhibition in the presence of EDTA, dithiothreitol, and 2-mercaptoethanol | Talaromyces amestolkiae | |
3.2.1.37 | Ni2+ | about 75% residual activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | Pb2+ | about 90% residual activity at 5 mM in citrate buffer | Talaromyces amestolkiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 0.17 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 0.2 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 0.2 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 0.22 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 0.22 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 0.48 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 3.6 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 5.8 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.37 | Al3+ | about 120% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | Co2+ | about 110% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | Fe2+ | about 125% activity at 5 mM in acetate buffer | Talaromyces amestolkiae | |
3.2.1.37 | Mg2+ | about 110% activity at 5 mM in acetate buffer | Talaromyces amestolkiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 200000 | - |
gel filtration | Talaromyces amestolkiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.37 | Talaromyces amestolkiae | A0A0H3W535 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.37 | glycoprotein | - |
Talaromyces amestolkiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.37 | HiTrap column chromatography, Mono S column chromatography, and Superose 12 gel filtration | Talaromyces amestolkiae |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.37 | mycelium | - |
Talaromyces amestolkiae | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.37 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.37 | 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + L-arabinopyranose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Talaromyces amestolkiae | 4-nitrophenol + D-xylopyranose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + xylobiose | - |
Talaromyces amestolkiae | 4-nitrophenol + xylotriose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + xylotriose | - |
Talaromyces amestolkiae | 4-nitrophenol + xylotetraose | - |
? | |
3.2.1.37 | beechwood xylan + H2O | - |
Talaromyces amestolkiae | D-xylose + ? | - |
? | |
3.2.1.37 | additional information | no activity with 4-nitrophenyl beta-D-glucopyranoside, 2-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 4-nitrophenyl beta-D-galactopyranoside, and 4-nitrophenyl beta-D-fucopyranoside | Talaromyces amestolkiae | ? | - |
? | |
3.2.1.37 | xylan + H2O | - |
Talaromyces amestolkiae | ? | - |
? | |
3.2.1.37 | xylobiose + H2O | - |
Talaromyces amestolkiae | 2 D-xylose | - |
? | |
3.2.1.37 | xylohexaose + H2O | - |
Talaromyces amestolkiae | 6 D-xylose | - |
? | |
3.2.1.37 | xylopentaose + H2O | - |
Talaromyces amestolkiae | 5 D-xylose | - |
? | |
3.2.1.37 | xylotetraose + 3 H2O | - |
Talaromyces amestolkiae | 4 D-xylose | - |
? | |
3.2.1.37 | xylotriose + H2O | - |
Talaromyces amestolkiae | 3 D-xylose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.37 | homodimer | 2 * 100000, SDS-PAGE | Talaromyces amestolkiae |
3.2.1.37 | homodimer | 2 * 102275, MALDI-TOF mass spectrometry | Talaromyces amestolkiae |
3.2.1.37 | homodimer | 2 * 84374, calculated from amino acid sequence | Talaromyces amestolkiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.37 | beta-xylosidase | - |
Talaromyces amestolkiae |
3.2.1.37 | BxTW1 | - |
Talaromyces amestolkiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 70 | - |
- |
Talaromyces amestolkiae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 50 | 70 | more than 50% activity between 50 and 70°C | Talaromyces amestolkiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 30 | 50 | the enzyme retains more than 70% activity and more than 50% activity after 72 h at 30-40 or 50°C, respectively | Talaromyces amestolkiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 32 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 39.2 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 51.2 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 66.1 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 143 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 173 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 183 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 220 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 229 | - |
beechwood xylan | at pH 3.0 and 50°C | Talaromyces amestolkiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 3 | - |
- |
Talaromyces amestolkiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 2.2 | 5.5 | about 95% activity at pH 2.2, 100% activity at pH 3.0, about 90% activity at pH 4.0, about 55% activity at pH 5.5, about 42% activity at pH 6.0, about 12% activity at pH 7.0 | Talaromyces amestolkiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 2.2 | 9 | the enzyme retains more than 80% activity after 72 h at pH 2.2-9.0 | Talaromyces amestolkiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 1.7 | - |
D-xylose | pH and temperature not specified in the publication | Talaromyces amestolkiae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.37 | Talaromyces amestolkiae | isoelectric focusing | - |
7.6 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.2.1.37 | Talaromyces amestolkiae | the expression of the enzyme can be induced by 1% (w/v) Avicel, 1% (w/v) D-xylose, or 1-3% (w/v) beechwood xylan | up |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 25 | - |
4-nitrophenyl alpha-L-arabinofuranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 62 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 140 | - |
xylohexaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 200 | - |
xylopentaose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 260 | - |
xylotetraose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 290 | - |
xylotriose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 380 | - |
xylobiose | at pH 3.0 and 50°C | Talaromyces amestolkiae | |
3.2.1.37 | 1000 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 3.0 and 50°C | Talaromyces amestolkiae |