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Literature summary extracted from

  • Li, Z.; Wu, J.; Zhang, B.; Wang, F.; Ye, X.; Huang, Y.; Huang, Q.; Cui, Z.
    AmyM, a novel maltohexaose-forming alpha-amylase from Corallococcus sp. strain EGB (2015), Appl. Environ. Microbiol., 81, 1977-1987 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.98 Triton X-100 30 mg/ml, 1.2fold activation Corallococcus sp. EGB

Application

EC Number Application Comment Organism
3.2.1.98 industry applications in starch processing technologies because of its highly specific activity, halotolerance, unique substrate specificity, and end product pattern Corallococcus sp. EGB

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.98 expression in Escherichia coli BL21(DE3) with a hexahistidine tag Corallococcus sp. EGB

General Stability

EC Number General Stability Organism
3.2.1.98 the enzyme is highly tolerant to high concentrations of salts, detergents, and various organic solvents Corallococcus sp. EGB
3.2.1.98 the enzyme is still active in the presence of high salt concentrations, with 74% and 88% of its activity being retained in 1 M NaCl and KCl solutions, respectively, and 67% of its activity being retained in a 3 M KCl solution. The enzymatic activity is reduced when the NaCl concentration is 2 M Corallococcus sp. EGB

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.98 acetonitrile 20%, 38.6% loss of activity Corallococcus sp. EGB
3.2.1.98 Ba2+ 1 mM, 11.1% loss of activity Corallococcus sp. EGB
3.2.1.98 Co2+ 1 mM, 68.3% loss of activity Corallococcus sp. EGB
3.2.1.98 Cr3+ 1 mM, 10.1% loss of activity Corallococcus sp. EGB
3.2.1.98 Cu+ 1 mM, 83.1% loss of activity Corallococcus sp. EGB
3.2.1.98 Cu2+ 1 mM, 89.8% loss of activity Corallococcus sp. EGB
3.2.1.98 DMSO 10%, 18.3% loss of activity Corallococcus sp. EGB
3.2.1.98 EDTA 10 mM, 83% loss of activity Corallococcus sp. EGB
3.2.1.98 ethanol 20%, 29.5% loss of activity Corallococcus sp. EGB
3.2.1.98 Fe2+ 1 mM, 74.7% loss of activity Corallococcus sp. EGB
3.2.1.98 Fe3+ 1 mM, 85.9% loss of activity Corallococcus sp. EGB
3.2.1.98 methanol 20%, 22.4% loss of activity Corallococcus sp. EGB
3.2.1.98 Mn2+ 1 mM, 35.2% loss of activity Corallococcus sp. EGB
3.2.1.98 Ni2+ 1 mM, 59.7% loss of activity Corallococcus sp. EGB
3.2.1.98 SDS 2 mg/ml, 46.5% loss of activity Corallococcus sp. EGB
3.2.1.98 Zn2+ 1 mM, 91.9% loss of activity Corallococcus sp. EGB

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.98 additional information
-
 soluble starch Km-value: 6.61 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.98 Ca2+ 1 mM, activation to 109% of control Corallococcus sp. EGB
3.2.1.98 K+ 1 mM, activation to 127% of control Corallococcus sp. EGB
3.2.1.98 Mg2+ 1 mM, activation to 117% of control Corallococcus sp. EGB

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.98 43000
-
 SDS-PAGE Corallococcus sp. EGB
3.2.1.98 55460
-
 calculated from sequence Corallococcus sp. EGB

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Corallococcus sp. EGB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.98 Ni-nitrilotriacetic acid affinity chromatography Corallococcus sp. EGB

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylopectin + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 amylose + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 corn starch + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 gamma-dextrin + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 glycogen + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 additional information no reaction is observed for pullulan, dextran, and alpha- or beta-cyclodextrins Corallococcus sp. EGB ?
-
 ?
3.2.1.98 soluble starch + H2O maltohexaose accounts for 59.4% of the maltooligosaccharides produced. Glucose (3.9%), maltose (6.2%), maltotriose (8.1%), maltotetraose (8.3%), maltopentaose (8.8%) and maltoheptaose (5.3%) Corallococcus sp. EGB maltohexaose + maltopentaose + maltose + D-glucose + maltotriose + maltotetraose
-
 ?
3.2.1.98 sweet potato starch + H2O
-
 Corallococcus sp. EGB ?
-
 ?
3.2.1.98 waxy maize starch + H2O
-
 Corallococcus sp. EGB maltohexaose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.98 ? x * 43000, SDS-PAGE Corallococcus sp. EGB

Synonyms

EC Number Synonyms Comment Organism
3.2.1.98 AmyM
-
 Corallococcus sp. EGB

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.98 50
-
 optimally active at 50°C in Tris-HCl buffer (50 mM, pH 7.0) Corallococcus sp. EGB

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.98 40 70 40°C: about 50% of maximal activity, 70°C: about 30% of maximal activity Corallococcus sp. EGB

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.98 50
-
 stable below Corallococcus sp. EGB

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.98 7
-
-
 Corallococcus sp. EGB

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.98 5 9 pH 5.0: about 30% of maximal activity, pH 9.0: about 40% of maximal activity Corallococcus sp. EGB

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.98 5 10 stable Corallococcus sp. EGB

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.98 Corallococcus sp. EGB calculated from sequence
-
 6.45