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Literature summary extracted from

  • Cafaro, V.; Izzo, V.; Scognamiglio, R.; Notomista, E.; Capasso, P.; Casbarra, A.; Pucci, P.; Di Donato, A.
    Phenol hydroxylase and toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1 interplay between two enzymes (2004), Appl. Environ. Microbiol., 70, 2211-2219 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.244 expression in Escherichia coli Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.244 0.00061
-
 phenol 25°C, pH not specified in the publication Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.244 Pseudomonas stutzeri Q84AQ4 and Q84AQ3 and Q84AQ2 and Q84AQ1 and Q84AQ0 Q84AQ4 i.e. subunit PhL, Q84AQ3 i.e. subunit PhM, Q84AQ2 i.e. subunit PhN, Q84AQ1 i.e. subunit PhO, Q84AQ0 i.e. subunit PhP
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.244 benzene + NADH + H+ + O2
-
 Pseudomonas stutzeri phenol + NAD+ + H2O
-
 ?
1.14.13.244 phenol + NADH + H+ + O2
-
 Pseudomonas stutzeri catechol + NAD+ + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.244 0.286
-
 phenol 25°C, pH not specified in the publication Pseudomonas stutzeri

General Information

EC Number General Information Comment Organism
1.14.13.244 physiological function the PhP component transfers electrons from NADH to a subcomplex endowed with hydroxylase activity. Subunit PhM displays a regulatory function. Cupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules Pseudomonas stutzeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.244 469
-
 phenol 25°C, pH not specified in the publication Pseudomonas stutzeri