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Literature summary extracted from

  • Haque, M.; Hong, S.; Hwang, C.; Kim, S.; Cho, K.
    Cloning of an organophosphorus hydrolase (opdD) gene of Lactobacillus sakei WCP904 isolated from chlorpyrifos-impregnated kimchi and hydrolysis activities of its gene product for organophosphorus pesticides (2018), Appl. Biol. Chem., 61, 643-651 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.1 gene opdD, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3) Latilactobacillus sakei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.8.1 cadusafos + H2O Latilactobacillus sakei
-
 O,O-diethylphosphorodithioate + phenol
-
 ?
3.1.8.1 cadusafos + H2O Latilactobacillus sakei WCP904
-
 O,O-diethylphosphorodithioate + phenol
-
 ?
3.1.8.1 chlorpyrifos + H2O Latilactobacillus sakei
-
 O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
-
 ?
3.1.8.1 chlorpyrifos + H2O Latilactobacillus sakei WCP904
-
 O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
-
 ?
3.1.8.1 coumaphos + H2O Latilactobacillus sakei
-
 O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
-
 ?
3.1.8.1 coumaphos + H2O Latilactobacillus sakei WCP904
-
 O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
-
 ?
3.1.8.1 diazinon + H2O Latilactobacillus sakei
-
 O,O-diethylphosphorothioate + ?
-
 ?
3.1.8.1 diazinon + H2O Latilactobacillus sakei WCP904
-
 O,O-diethylphosphorothioate + ?
-
 ?
3.1.8.1 diethyl-parathion + H2O Latilactobacillus sakei
-
 diethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 dimethyl-parathion + H2O Latilactobacillus sakei
-
 dimethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 dyfonate + H2O Latilactobacillus sakei
-
 O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol
-
 ?
3.1.8.1 ethoprophos + H2O Latilactobacillus sakei
-
 ?
-
 ?
3.1.8.1 fenamiphos + H2O Latilactobacillus sakei
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Latilactobacillus sakei
-
 isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos
-
3.1.8.1 Latilactobacillus sakei WCP904
-
 isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.1 recombinant enzyme from Escherichia coli strain BL21(DE3) Latilactobacillus sakei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.1 additional information
-
 activity in wild-type strain WCP904 and of purified recombinant enzyme with different organophosphorus pesticides, overview Latilactobacillus sakei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 4-nitrophenyl butyrate + H2O
-
 Latilactobacillus sakei 4-nitrophenol + butyrate
-
 ?
3.1.8.1 4-nitrophenyl butyrate + H2O
-
 Latilactobacillus sakei WCP904 4-nitrophenol + butyrate
-
 ?
3.1.8.1 cadusafos + H2O
-
 Latilactobacillus sakei O,O-diethylphosphorodithioate + phenol
-
 ?
3.1.8.1 cadusafos + H2O
-
 Latilactobacillus sakei WCP904 O,O-diethylphosphorodithioate + phenol
-
 ?
3.1.8.1 chlorpyrifos + H2O
-
 Latilactobacillus sakei O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
-
 ?
3.1.8.1 chlorpyrifos + H2O
-
 Latilactobacillus sakei WCP904 O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
-
 ?
3.1.8.1 coumaphos + H2O
-
 Latilactobacillus sakei O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
-
 ?
3.1.8.1 coumaphos + H2O
-
 Latilactobacillus sakei WCP904 O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
-
 ?
3.1.8.1 diazinon + H2O
-
 Latilactobacillus sakei O,O-diethylphosphorothioate + ?
-
 ?
3.1.8.1 diazinon + H2O
-
 Latilactobacillus sakei WCP904 O,O-diethylphosphorothioate + ?
-
 ?
3.1.8.1 diethyl-parathion + H2O
-
 Latilactobacillus sakei diethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 dimethyl-parathion + H2O
-
 Latilactobacillus sakei dimethyl thiophosphate + 4-nitrophenol
-
 ?
3.1.8.1 dyfonate + H2O
-
 Latilactobacillus sakei O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol
-
 ?
3.1.8.1 ethoprophos + H2O
-
 Latilactobacillus sakei ?
-
 ?
3.1.8.1 fenamiphos + H2O
-
 Latilactobacillus sakei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.8.1 ? x * 31000, recombinant enzyme, SDS-PAGE Latilactobacillus sakei
3.1.8.1 More the deduced amino acid sequences of OpdD shows a potential serine hydrolase motif such as G-H-S116-Q-G Latilactobacillus sakei

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 OpdD
-
 Latilactobacillus sakei
3.1.8.1 organophosphorus hydrolase
-
 Latilactobacillus sakei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.8.1 30
-
 recombinant enzyme, with substrate 4-nitrophenyl butyrate Latilactobacillus sakei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.8.1 6
-
 recombinant enzyme, with substrate 4-nitrophenyl butyrate Latilactobacillus sakei

General Information

EC Number General Information Comment Organism
3.1.8.1 additional information the deduced amino acid sequences of OpdD show a potential serine hydrolase motif such as G-H-S116-Q-G Latilactobacillus sakei