Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shetty, P.
    Glucoamylase from the predacious fungus Arthrobotrys conoides a cationic enzyme with high debranching activity and raw starch digestibility (2016), Appl. Biochem. Microbiol., 52, 176-182 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 acarbose 84% inhibition at 0.004 mg/ml, 66% at 0.002 mg/ml Arthrobotrys conoides
3.2.1.3 Cu2+
-
 Arthrobotrys conoides
3.2.1.3 diethyl dicarbonate 48% inhibition at 4 mM, 76% at 10 mM Arthrobotrys conoides
3.2.1.3 EDTA 51% inhibition at 2 mM Arthrobotrys conoides
3.2.1.3 Hg2+ 46% inhibition at 0.002 mM Arthrobotrys conoides
3.2.1.3 N-bromosuccinimide 78% inhibition at 0.01 mM Arthrobotrys conoides
3.2.1.3 Pb2+
-
 Arthrobotrys conoides
3.2.1.3 Periodate 27% inhibition at 5 mM, 30% at 10 mM, 35% at 15 mM. 34% Glycosyl content of the enzyme is lost at 2.1 mM of periodate Arthrobotrys conoides
3.2.1.3 Rose bengal 48% inhibition at 0.25 mg/ml Arthrobotrys conoides
3.2.1.3 Zn2+
-
 Arthrobotrys conoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information KM values for amylopectin, glycogen and starch are 0.056, 0.062 and 0.065 mg/ml, respectively Arthrobotrys conoides
3.2.1.196 additional information
-
 amylopectin KM value 0.056 mg/ml, pH 7.0, 25°C Arthrobotrys conoides
3.2.1.196 additional information
-
 glycogen KM value 0.062 mg/ml, pH 7.0, 25°C Arthrobotrys conoides
3.2.1.196 additional information
-
 starch KM value 0.065 mg/ml, pH 7.0, 25°C Arthrobotrys conoides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Arthrobotrys conoides
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ca2+ 23% activation at 4 mM Arthrobotrys conoides
3.2.1.3 additional information Mg2+ and Mn2+ have negligible effect on the enzyme activity Arthrobotrys conoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 87000
-
 native enzyme, gel filtration Arthrobotrys conoides
3.2.1.196 87000
-
 gel filtration Arthrobotrys conoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Arthrobotrys conoides
-
 D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Arthrobotrys conoides
-
-
-
3.2.1.196 Arthrobotrys conoides
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein carbohydrate content of the enzyme is 26.3%, 34% glycosyl content is lost at 2.1 mM of periodate Arthrobotrys conoides

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native enzyme 68.2% by dialysis, gel filtration and anion exchange chromatography, more than 90% of native GA A binds to raw starch Arthrobotrys conoides
3.2.1.196 purification from culture filtrate Arthrobotrys conoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 165.8
-
 purified native enzyme, pH 5.6, 50°C Arthrobotrys conoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylose + H2O best substrate Arthrobotrys conoides D-glucose + ?
-
 ?
3.2.1.3 additional information the enzyme shows high affinity for the branched polysaccharides. It can digest raw starch Arthrobotrys conoides ?
-
 ?
3.2.1.3 starch + H2O
-
 Arthrobotrys conoides D-glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble and raw starch Arthrobotrys conoides D-glucose + ?
-
 ?
3.2.1.196 amylopectin + H2O
-
 Arthrobotrys conoides ?
-
 ?
3.2.1.196 glycogen + H2O
-
 Arthrobotrys conoides ?
-
 ?
3.2.1.196 raw starch + H2O
-
 Arthrobotrys conoides ?
-
 ?
3.2.1.196 starch + H2O
-
 Arthrobotrys conoides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer 1 * 83500, native enzyme, SDS-PAGE Arthrobotrys conoides
3.2.1.196 monomer 1 * 83500 SDS-PAGE Arthrobotrys conoides

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GA A
-
 Arthrobotrys conoides
3.2.1.3 glycoamylase
-
 Arthrobotrys conoides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
-
 Arthrobotrys conoides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 40
-
 purified native enzyme, 30 min, stable Arthrobotrys conoides
3.2.1.3 45
-
 purified native enzyme, 60 min, 59% activity remaining Arthrobotrys conoides
3.2.1.196 40
-
 stable for 30 min Arthrobotrys conoides
3.2.1.196 45
-
 1 h, 59% residual activity Arthrobotrys conoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5.4 5.8
-
 Arthrobotrys conoides
3.2.1.196 5.4 5.8
-
 Arthrobotrys conoides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3 8 activity range, profile overview Arthrobotrys conoides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 4.8 9 purified native enzyme, stable at Arthrobotrys conoides
3.2.1.196 4.8 9
-
 Arthrobotrys conoides