BRENDA - Enzyme Database

Penicillium purpurogenum produces a set of endoxylanases identification, heterologous expression, and characterization of a fourth xylanase, XynD, a novel enzyme belonging to glycoside hydrolase family 10

Echeverria, V.; Eyzaguirre, J.; Appl. Biochem. Biotechnol. 187, 298-309 (2019) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.8
gene xynD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted
Talaromyces purpureogenus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.8
Talaromyces purpureogenus
A0A2H4RAC5
i.e. Penicillium purpurogenum
-
3.2.1.8
Talaromyces purpureogenus MYA-38
A0A2H4RAC5
i.e. Penicillium purpurogenum
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.8
glycoprotein
the recombinant enzyme may be glycosylated
Talaromyces purpureogenus
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.8
recombinant extracellular enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and again ultrafiltration
Talaromyces purpureogenus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.8
47.6
-
recombinant enzyme from concentrated cell culture supernatant, pH 7.0, 60°C
Talaromyces purpureogenus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.8
beechwood xylan + H2O
best substrate
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
beechwood xylan + H2O
best substrate
749635
Talaromyces purpureogenus MYA-38
xylobiose
-
-
-
?
3.2.1.8
birchwood xylan + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
additional information
the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs
749635
Talaromyces purpureogenus
?
-
-
-
?
3.2.1.8
additional information
the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs
749635
Talaromyces purpureogenus MYA-38
?
-
-
-
?
3.2.1.8
oat spelt xylan + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
wheat arabinoxylan + H2O
best substrate
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
xylobiose + H2O
low activity
749635
Talaromyces purpureogenus
xylose
-
-
-
?
3.2.1.8
xylobiose + H2O
low activity
749635
Talaromyces purpureogenus MYA-38
xylose
-
-
-
?
3.2.1.8
xylopentaose + H2O
-
749635
Talaromyces purpureogenus
xylobiose + xylose
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
749635
Talaromyces purpureogenus MYA-38
xylobiose
-
-
-
?
3.2.1.8
xylotriose + H2O
-
749635
Talaromyces purpureogenus
xylobiose + xylose
-
-
-
?
3.2.1.8
xylotriose + H2O
-
749635
Talaromyces purpureogenus MYA-38
xylobiose + xylose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.8
?
x * 40997, sequence calculation, x * 60000, recombinant enzyme, SDS-PAGE
Talaromyces purpureogenus
3.2.1.8
More
the enzyme XynD consists of 387 amino acid residues with an N-terminal catalytic module, a linker rich in Ser and Thr residues, and a C-terminal family 1 carbohydrate-binding module
Talaromyces purpureogenus
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.8
beta-xylanase
UniProt
Talaromyces purpureogenus
3.2.1.8
endoxylanase
-
Talaromyces purpureogenus
3.2.1.8
XynD
-
Talaromyces purpureogenus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
60
-
recombinant enzyme
Talaromyces purpureogenus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.2.1.8
40
90
recombinant enzyme, over 80% of maximal activity within this range
Talaromyces purpureogenus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.8
40
-
purified recombinant enzyme, pH 6.5, 24 h, completely stable
Talaromyces purpureogenus
3.2.1.8
60
-
purified recombinant enzyme, pH 6.5, 60 min, stable
Talaromyces purpureogenus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
6.5
8.5
recombinant enzyme
Talaromyces purpureogenus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.8
7
9
recombinant enzyme, over 70% of maximal activity within this range
Talaromyces purpureogenus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.8
6
8
purified recombinant enzyme, 37°C, 24 h, 80% activity remaining
Talaromyces purpureogenus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.8
Talaromyces purpureogenus
sequence calculation
-
5.64
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
gene xynD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris strain GS115, the enzyme is secreted
Talaromyces purpureogenus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.8
glycoprotein
the recombinant enzyme may be glycosylated
Talaromyces purpureogenus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
recombinant extracellular enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and again ultrafiltration
Talaromyces purpureogenus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.8
47.6
-
recombinant enzyme from concentrated cell culture supernatant, pH 7.0, 60°C
Talaromyces purpureogenus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.8
beechwood xylan + H2O
best substrate
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
beechwood xylan + H2O
best substrate
749635
Talaromyces purpureogenus MYA-38
xylobiose
-
-
-
?
3.2.1.8
birchwood xylan + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
additional information
the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs
749635
Talaromyces purpureogenus
?
-
-
-
?
3.2.1.8
additional information
the enzyme acts as an endoxylanase. The enzyme is also active on xylooligosaccharides, main product is xylobiose and very little xylose is produced. No transglycosylation occurs
749635
Talaromyces purpureogenus MYA-38
?
-
-
-
?
3.2.1.8
oat spelt xylan + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
wheat arabinoxylan + H2O
best substrate
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
xylobiose + H2O
low activity
749635
Talaromyces purpureogenus
xylose
-
-
-
?
3.2.1.8
xylobiose + H2O
low activity
749635
Talaromyces purpureogenus MYA-38
xylose
-
-
-
?
3.2.1.8
xylopentaose + H2O
-
749635
Talaromyces purpureogenus
xylobiose + xylose
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
749635
Talaromyces purpureogenus
xylobiose
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
749635
Talaromyces purpureogenus MYA-38
xylobiose
-
-
-
?
3.2.1.8
xylotriose + H2O
-
749635
Talaromyces purpureogenus
xylobiose + xylose
-
-
-
?
3.2.1.8
xylotriose + H2O
-
749635
Talaromyces purpureogenus MYA-38
xylobiose + xylose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.8
?
x * 40997, sequence calculation, x * 60000, recombinant enzyme, SDS-PAGE
Talaromyces purpureogenus
3.2.1.8
More
the enzyme XynD consists of 387 amino acid residues with an N-terminal catalytic module, a linker rich in Ser and Thr residues, and a C-terminal family 1 carbohydrate-binding module
Talaromyces purpureogenus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
60
-
recombinant enzyme
Talaromyces purpureogenus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.2.1.8
40
90
recombinant enzyme, over 80% of maximal activity within this range
Talaromyces purpureogenus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.8
40
-
purified recombinant enzyme, pH 6.5, 24 h, completely stable
Talaromyces purpureogenus
3.2.1.8
60
-
purified recombinant enzyme, pH 6.5, 60 min, stable
Talaromyces purpureogenus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
6.5
8.5
recombinant enzyme
Talaromyces purpureogenus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.8
7
9
recombinant enzyme, over 70% of maximal activity within this range
Talaromyces purpureogenus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.8
6
8
purified recombinant enzyme, 37°C, 24 h, 80% activity remaining
Talaromyces purpureogenus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.8
Talaromyces purpureogenus
sequence calculation
-
5.64
General Information
EC Number
General Information
Commentary
Organism
3.2.1.8
evolution
enzyme XynD belongs to the glycoside hydrolase family 10, GH10
Talaromyces purpureogenus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.2.1.8
evolution
enzyme XynD belongs to the glycoside hydrolase family 10, GH10
Talaromyces purpureogenus