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Literature summary extracted from

  • Leema Roseline, T.; Sachindra, N.M.
    Purification and characterization of agarase from marine bacteria Acinetobacter sp. PS12B and its use for preparing bioactive hydrolysate from agarophyte red seaweed Gracilaria verrucosa (2018), Appl. Biochem. Biotechnol., 186, 66-84 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B1 2-mercaptoethanol about 135% activity at 10 mM Acinetobacter junii
3.2.1.B1 dithiothreitol about 145% activity at 10 mM Acinetobacter junii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B1 acetone 86.9% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 DMSO 79.3% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 EDTA EDTA reduces the agarase activity by 58% at 5 mM Acinetobacter junii
3.2.1.B1 ethanol 76.9% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 ethyl acetate 12.1% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 hexane 76.9% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 methanol 72.6% residual activity at 5% (v/v) Acinetobacter junii
3.2.1.B1 SDS about 83% residual activity at 5 mM Acinetobacter junii
3.2.1.B1 Urea about 80% residual activity at 5 mM Acinetobacter junii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
 Acinetobacter junii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B1 Fe2+ 133.4% activity at 10 mM Acinetobacter junii
3.2.1.B1 Mn2+ 134.4% activity at 5 mM Acinetobacter junii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Acinetobacter junii
-
-
-
3.2.1.B1 Acinetobacter junii PS12B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 ammonium sulfate precipitation and DEAE-cellulose column chromatography Acinetobacter junii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 agar + H2O
-
 Acinetobacter junii neoagarotriose + neoagarohexaose + neoagaroheptaose
-
 ?
3.2.1.B1 agar + H2O
-
 Acinetobacter junii PS12B neoagarotriose + neoagarohexaose + neoagaroheptaose
-
 ?
3.2.1.B1 seaweed + H2O
-
 Acinetobacter junii ?
-
 ?
3.2.1.B1 seaweed + H2O
-
 Acinetobacter junii PS12B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 ? x * 24000, SDS-PAGE Acinetobacter junii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B1 endo-acting beta-agarase
-
 Acinetobacter junii
3.2.1.B1 endo-type beta-agarase
-
 Acinetobacter junii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 40
-
-
 Acinetobacter junii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B1 50
-
 the enzyme is stable up to 1 h at 50°C Acinetobacter junii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 8
-
-
 Acinetobacter junii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B1 4 8 the purified enzyme is most stable at pH 7.0-8.0 with more than 98% residual activity after 24 h. The residual activity of enzyme reduces to less than 50% when incubated for 24 h at pH 4.0 Acinetobacter junii