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Literature summary extracted from

  • Bermudez-Garcia, E.; Pena-Montes, C.; Castro-Rodriguez, J.A.; Gonzalez-Canto, A.; Navarro-Ocana, A.; Farres, A.
    ANCUT2, a thermo-alkaline cutinase from Aspergillus nidulans and its potential applications (2017), Appl. Biochem. Biotechnol., 182, 1014-1036 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 Ca2+ 1 mM, 47% residual acivity Aspergillus nidulans
3.1.1.74 Cu2+ 10 mM, 52% residual acivity Aspergillus nidulans
3.1.1.74 EDTA 10 mM, 42% residual acivity Aspergillus nidulans
3.1.1.74 Fe3+ 10 mM, 43% residual acivity Aspergillus nidulans
3.1.1.74 K+ 1 mM, 42% residual acivity Aspergillus nidulans
3.1.1.74 Mg2+ 1 mM, 42% residual acivity Aspergillus nidulans
3.1.1.74 Na+ 1 mM, 39% residual acivity Aspergillus nidulans
3.1.1.74 PMSF 10 mM, 47% residual acivity Aspergillus nidulans
3.1.1.74 SDS 10 mM, 47% residual acivity Aspergillus nidulans
3.1.1.74 Tween 80 10 mM, 48% residual acivity Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 6.88
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.74 Acetone 30%, 44% residual activity Aspergillus nidulans
3.1.1.74 DMSO 30%, 53% residual activity Aspergillus nidulans
3.1.1.74 Ethanol 30%, 45% residual activity Aspergillus nidulans
3.1.1.74 hexane 30%, 53% residual activity Aspergillus nidulans
3.1.1.74 isopropanol 30%, 46% residual activity Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Aspergillus nidulans Q5AVY9
-
-
3.1.1.74 Aspergillus nidulans ATCC 38163 Q5AVY9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74
-
 Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans ATCC 38163 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl butanoate + H2O about 55% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl butanoate + H2O about 55% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl myristate + H2O about 50% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + myristate
-
 ?
3.1.1.74 4-nitrophenyl myristate + H2O about 50% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + myristate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O about 30% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O about 30% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl stearate + H2O about 28% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + stearate
-
 ?
3.1.1.74 cutin + H2O
-
 Aspergillus nidulans palmitate + ?
-
 ?
3.1.1.74 additional information enzyme preferably hydrolyzes short-chain length esters (C2-C4), but it also displays a slight affinity for long-chain length esters (C14-C18) Aspergillus nidulans ?
-
 ?
3.1.1.74 additional information enzyme preferably hydrolyzes short-chain length esters (C2-C4), but it also displays a slight affinity for long-chain length esters (C14-C18) Aspergillus nidulans ATCC 38163 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 ? x * 29000, SDS-PAGE, x * 24350, calculated from sequence, mature protein Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 Cut2
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 60
-
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 40
-
 half-life 407 min Aspergillus nidulans
3.1.1.74 50
-
 half-life 110 min Aspergillus nidulans
3.1.1.74 60
-
 1h, pH 6-10, 60% residual activity Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.74 40.3
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 9
-
-
 Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.74 6 10 1h, 60°C, 60% residual activity Aspergillus nidulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.74 Aspergillus nidulans calculated from sequence
-
 4.9
3.1.1.74 Aspergillus nidulans isoelectric focusing
-
 5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.74 5.87
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans