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Literature summary extracted from

  • Ranjan, B.; Singh, B.; Satyanarayana, T.
    Characteristics of recombinant phytase (rSt-Phy) of the thermophilic mold Sporotrichum thermophile and its applicability in dephytinizing foods (2015), Appl. Biochem. Biotechnol., 177, 1753-1766 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 butanol slight activation Thermothelomyces thermophilus
3.1.3.8 isoamyl alcohol slight activation Thermothelomyces thermophilus
3.1.3.8 additional information poor activation by hexane Thermothelomyces thermophilus
3.1.3.8 Tween 20 34% activation at 0.5% Thermothelomyces thermophilus
3.1.3.8 Tween 40 42% activation at 0.5% Thermothelomyces thermophilus
3.1.3.8 Tween 80 67.5% activation at 0.5% Thermothelomyces thermophilus

Application

EC Number Application Comment Organism
3.1.3.8 food industry the recombinant enzyme rSt-Phy is useful in dephytinization of tandoori and naan (unleavened flat Indian breads), and bread, liberating soluble inorganic phosphate that mitigates anti-nutrient effects of phytic acid Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene PHYA, construction of rSt-Phy gene using overlap extension PCR for removal of an intron, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 2,3-Butanedione
-
Thermothelomyces thermophilus
3.1.3.8 Al3+
-
Thermothelomyces thermophilus
3.1.3.8 benzyl alcohol slight inhibition Thermothelomyces thermophilus
3.1.3.8 Ca2+ slight inhibition Thermothelomyces thermophilus
3.1.3.8 Co2+ slight inhibition Thermothelomyces thermophilus
3.1.3.8 Cu2+
-
Thermothelomyces thermophilus
3.1.3.8 dithiothreitol slight inhibition Thermothelomyces thermophilus
3.1.3.8 ethanol slight inhibition Thermothelomyces thermophilus
3.1.3.8 Fe2+
-
Thermothelomyces thermophilus
3.1.3.8 Fe3+
-
Thermothelomyces thermophilus
3.1.3.8 guanidinium hydrochloride
-
Thermothelomyces thermophilus
3.1.3.8 iodoacetate slight inhibition Thermothelomyces thermophilus
3.1.3.8 Mn2+
-
Thermothelomyces thermophilus
3.1.3.8 additional information the enzyme is resistent against pepsin and trypsin, 95% of initial activity is retrieved after 1.0-h incubation in pepsin/trypsin. Poor inhibition by acetone, EDTA and 2-mercaptoethanol Thermothelomyces thermophilus
3.1.3.8 N-bromosuccinimide slight inhibition Thermothelomyces thermophilus
3.1.3.8 N-ethylmaleimide slight inhibition Thermothelomyces thermophilus
3.1.3.8 Na+ slight inhibition Thermothelomyces thermophilus
3.1.3.8 PMSF
-
Thermothelomyces thermophilus
3.1.3.8 potassium iodide
-
Thermothelomyces thermophilus
3.1.3.8 SDS slight inhibition at 0.5% Thermothelomyces thermophilus
3.1.3.8 Toluene slight inhibition Thermothelomyces thermophilus
3.1.3.8 Urea
-
Thermothelomyces thermophilus
3.1.3.8 Woodward's reagent K
-
Thermothelomyces thermophilus
3.1.3.8 Zn2+
-
Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 additional information
-
additional information thermodynamics of the recombinant enzyme Thermothelomyces thermophilus
3.1.3.8 0.143
-
myo-inositol hexakisphosphate recombinant enzyme, pH 5.0, 60°C Thermothelomyces thermophilus
3.1.3.8 0.156
-
myo-inositol hexakisphosphate native enzyme, pH 5.0, 60°C Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ba2+ slight activation Thermothelomyces thermophilus
3.1.3.8 Mg2+ slight activation Thermothelomyces thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Thermothelomyces thermophilus
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O Thermothelomyces thermophilus ATCC 42464
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O Thermothelomyces thermophilus BCRC 31852
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O Thermothelomyces thermophilus DSM 1799
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Thermothelomyces thermophilus O00107 i.e. Sporotrichum thermophile or Thielavia heterothallica
-
3.1.3.8 Thermothelomyces thermophilus ATCC 42464 O00107 i.e. Sporotrichum thermophile or Thielavia heterothallica
-
3.1.3.8 Thermothelomyces thermophilus BCRC 31852 O00107 i.e. Sporotrichum thermophile or Thielavia heterothallica
-
3.1.3.8 Thermothelomyces thermophilus DSM 1799 O00107 i.e. Sporotrichum thermophile or Thielavia heterothallica
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Thermothelomyces thermophilus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.8 recombinant enzyme from Escherichia coli inclusion bodies is solubilized by 6 M urea and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0. The cell-free lysate is added to the equilibrated resins and is kept for 1 h on a rocker. The recombinant protein bound to the column is refolded on the column by passing five column volumes of buffer A (6 M urea and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0) and buffer B (20% v/v glycerol and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0) in a linear gradient of 6.0 to 0.0 M urea Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 52.4
-
purified native enzyme, pH 5.0, 60°C Thermothelomyces thermophilus
3.1.3.8 8000
-
purified recombinant enzyme, pH 5.0, 60°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Thermothelomyces thermophilus 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Thermothelomyces thermophilus ATCC 42464 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Thermothelomyces thermophilus BCRC 31852 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Thermothelomyces thermophilus DSM 1799 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 53000, sequence calculation, x * 55000, recombinant His-tagged enzyme, SDS-PAGE, x * 90000, native enzyme, SDS-PAGE Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 3-phytase A
-
Thermothelomyces thermophilus
3.1.3.8 PhyA
-
Thermothelomyces thermophilus
3.1.3.8 St-Phy
-
Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
recombinant and native enzymes Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
purified recombinant His-tagged enzyme, pH 5.0, has a half-life of 4 h. Purified native enzyme, pH 5.0, has a half-life of 16 h Thermothelomyces thermophilus
3.1.3.8 80
-
purified recombinant His-tagged enzyme, pH 5.0, has a half-life of 38 min. Purified native enzyme, pH 5.0, has a half-life of 1.5 h Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 5100
-
myo-inositol hexakisphosphate recombinant enzyme, pH 5.0, 60°C Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
recombinant and native enzymes Thermothelomyces thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Thermothelomyces thermophilus sequence calculation
-
5.05

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 242.3
-
myo-inositol hexakisphosphate native enzyme, pH 5.0, 60°C Thermothelomyces thermophilus
3.1.3.8 37207.6
-
myo-inositol hexakisphosphate recombinant enzyme, pH 5.0, 60°C Thermothelomyces thermophilus