Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yin, Y.R.; Xian, W.D.; Han, M.X.; Zhou, E.M.; Liu, L.; Alkhalifah, D.H.M.; Hozzein, W.N.; Xiao, M.; Li, W.J.
    Expression and characterisation of a pH and salt tolerant, thermostable and xylose tolerant recombinant GH43 beta-xylosidase from Thermobifida halotolerans YIM 90462T for promoting hemicellulose degradation (2019), Antonie van Leeuwenhoek, 112, 339-350 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 degradation the enzyme has potential for promoting hemicellulose degradation and other industrial applications Thermobifida halotolerans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 overexpression in Escherichia coli Thermobifida halotolerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.42
-
 p-nitrophenyl alpha-L-arabinoside 55°C, pH 7.0 Thermobifida halotolerans
3.2.1.37 0.78
-
 4-nitrophenyl beta-D-xyloside 55°C, pH 7.0 Thermobifida halotolerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 additional information activity is not affected by K+, Mg2+, Fe2+, Fe3+, Ca2+, Co2+, Ba2+ Thermobifida halotolerans
3.2.1.37 NaCl the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) Thermobifida halotolerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 61500
-
-
 Thermobifida halotolerans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Thermobifida halotolerans A0A399G7U0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Thermobifida halotolerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O the enzyme displays high catalytic activity (60%) in presence of 5-35% NaCl (w/v) or 1-20% ionic liquid (w/v) or 1-50 mM xylose Thermobifida halotolerans 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information no activity is found for beechwood xylan and cellobiose Thermobifida halotolerans ?
-
 ?
3.2.1.37 p-nitrophenyl alpha-L-arabinoside + H2O about 6% of the activity with 4-nitrophenyl beta-D-xylopyranoside Thermobifida halotolerans 4-nitrophenol + alpha-L-arabinose
-
 ?
3.2.1.37 xylobiose + H2O 25% of the activity with 4-nitrophenyl beta-D-xylopyranoside Thermobifida halotolerans 2 beta-D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O 21% of the activity with 4-nitrophenyl beta-D-xylopyranoside Thermobifida halotolerans xylotriose + beta-D-xylose
-
 ?
3.2.1.37 xylotriose + 2 H2O about 12.1% of the activity with 4-nitrophenyl beta-D-xylopyranoside Thermobifida halotolerans 3 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 61500 Thermobifida halotolerans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 Thxyl43A
-
 Thermobifida halotolerans
3.2.1.37 xyl43A
-
 Thermobifida halotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 55
-
-
 Thermobifida halotolerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 15 60 15°C: 27 % of maximal activity, 25°C: 40% of maximal activity, 60°C: 60% of maximal activity Thermobifida halotolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 40
-
 the enzyme retains more than 80% activity after incubation for 66 h Thermobifida halotolerans
3.2.1.37 50
-
 the enzyme retains more than 80% activity after incubation for 66 h Thermobifida halotolerans
3.2.1.37 55
-
 t1/2: 29.7 h. The enzyme retains 47% of its activity after 30 h incubation at 55°C, the enzyme retains 90% of its activity after 24 h incubation at 55°C Thermobifida halotolerans
3.2.1.37 60
-
 t1/2: 4.8 h Thermobifida halotolerans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 3.8
-
 p-nitrophenyl alpha-L-arabinoside 55°C, pH 7.0 Thermobifida halotolerans
3.2.1.37 50
-
 4-nitrophenyl beta-D-xyloside 55°C, pH 7.0 Thermobifida halotolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 7
-
-
 Thermobifida halotolerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4 10.6 the enzyme is notably active at pH 4-6 and pH 9-10. At pH 8.0 about 65% and 90% relative activity is detected in citrate buffer and borate buffer respectively. At pH 8.6 and 9.0 less than 65% relative activity is observed in borate buffer, while more than 85% is found in Gly-NaOH buffer. More than 60% relative activity is noted at pH 10.6 Thermobifida halotolerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 4 12 the enzyme retains more than 80% residual activity after incubation at pH range of 4.0 to 12.0 for 24 h Thermobifida halotolerans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 9
-
 p-nitrophenyl alpha-L-arabinoside 55°C, pH 7.0 Thermobifida halotolerans
3.2.1.37 64.1
-
 4-nitrophenyl beta-D-xyloside 55°C, pH 7.0 Thermobifida halotolerans