EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.18 | R152K | the mutation mediates reduced inhibition by oseltamivir, zanamivir, peramivir and laninamivir (10-100fold increase of IC50 value compared to the wild type enzyme) | unidentified influenza virus |
3.2.1.18 | R152K/V94I | the mutations mediate reduced inhibition by oseltamivir, zanamivir, peramivir and laninamivir (10-100fold increase of IC50 value compared to the wild type enzyme) | unidentified influenza virus |
3.2.1.18 | R368K | the mutation mediates reduced inhibition by oseltamivir, zanamivir and laninamivir (96-57fold increase of IC50 value compared to the wild type enzyme) as well as peramivir (172fold increase of IC50 value compared to the wild type enzyme) | unidentified influenza virus |
3.2.1.18 | V94IK | the mutant shows normal inhibition by oseltamivir, zanamivir, peramivir and laninamivir | unidentified influenza virus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.18 | 2,3-dehydro-2-deoxy-N-acetylneuraminic acid | weak inhibitor | unidentified influenza virus | |
3.2.1.18 | laninamivir | - |
unidentified influenza virus | |
3.2.1.18 | oseltamivir | - |
unidentified influenza virus | |
3.2.1.18 | peramivir | - |
unidentified influenza virus | |
3.2.1.18 | zanamivir | - |
unidentified influenza virus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.18 | 0.01506 | - |
4-methylumbelliferyl N-acetylneuraminic acid | mutant enzyme V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | |
3.2.1.18 | 0.02 | - |
4-methylumbelliferyl N-acetylneuraminic acid | wild type enzyme, at 37°C, pH not specified in the publication | unidentified influenza virus | |
3.2.1.18 | 0.2008 | - |
4-methylumbelliferyl N-acetylneuraminic acid | mutant enzyme R368K, at 37°C, pH not specified in the publication | unidentified influenza virus | |
3.2.1.18 | 0.2355 | - |
4-methylumbelliferyl N-acetylneuraminic acid | mutant enzyme R152K/V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | |
3.2.1.18 | 0.5652 | - |
4-methylumbelliferyl N-acetylneuraminic acid | mutant enzyme R152K, at 37°C, pH not specified in the publication | unidentified influenza virus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.18 | unidentified influenza virus | - |
- |
- |
3.2.1.18 | unidentified influenza virus A/Quebec/144147/09 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.18 | 4-methylumbelliferyl N-acetylneuraminic acid + H2O | - |
unidentified influenza virus | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? | |
3.2.1.18 | 4-methylumbelliferyl N-acetylneuraminic acid + H2O | - |
unidentified influenza virus A/Quebec/144147/09 | 4-methylumbelliferone + N-acetylneuraminic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.18 | neuraminidase | - |
unidentified influenza virus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.18 | 0.00016 | - |
mutant enzyme V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | peramivir | |
3.2.1.18 | 0.0002 | - |
wild type enzyme, at 37°C, pH not specified in the publication | unidentified influenza virus | peramivir | |
3.2.1.18 | 0.00036 | - |
wild type enzyme, at 37°C, pH not specified in the publication | unidentified influenza virus | laninamivir | |
3.2.1.18 | 0.00066 | - |
mutant enzyme V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | zanamivir | |
3.2.1.18 | 0.00068 | - |
wild type enzyme, at 37°C, pH not specified in the publication | unidentified influenza virus | zanamivir | |
3.2.1.18 | 0.0009 | - |
mutant enzyme V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | laninamivir | |
3.2.1.18 | 0.00142 | - |
mutant enzyme V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | oseltamivir | |
3.2.1.18 | 0.00148 | - |
wild type enzyme, at 37°C, pH not specified in the publication | unidentified influenza virus | oseltamivir | |
3.2.1.18 | 0.00181 | - |
mutant enzyme R152K, at 37°C, pH not specified in the publication | unidentified influenza virus | laninamivir | |
3.2.1.18 | 0.00649 | - |
mutant enzyme R152K/V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | laninamivir | |
3.2.1.18 | 0.00804 | - |
mutant enzyme R368K, at 37°C, pH not specified in the publication | unidentified influenza virus | laninamivir | |
3.2.1.18 | 0.0114 | - |
mutant enzyme R152K/V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | peramivir | |
3.2.1.18 | 0.0148 | - |
mutant enzyme R368K, at 37°C, pH not specified in the publication | unidentified influenza virus | peramivir | |
3.2.1.18 | 0.01768 | - |
mutant enzyme R368K, at 37°C, pH not specified in the publication | unidentified influenza virus | zanamivir | |
3.2.1.18 | 0.01776 | - |
mutant enzyme R152K/V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | zanamivir | |
3.2.1.18 | 0.0344 | - |
mutant enzyme R152K, at 37°C, pH not specified in the publication | unidentified influenza virus | peramivir | |
3.2.1.18 | 0.03936 | - |
mutant enzyme R152K, at 37°C, pH not specified in the publication | unidentified influenza virus | zanamivir | |
3.2.1.18 | 0.07812 | - |
mutant enzyme R152K/V94I, at 37°C, pH not specified in the publication | unidentified influenza virus | oseltamivir | |
3.2.1.18 | 0.10048 | - |
mutant enzyme R368K, at 37°C, pH not specified in the publication | unidentified influenza virus | oseltamivir | |
3.2.1.18 | 0.14252 | - |
mutant enzyme R152K, at 37°C, pH not specified in the publication | unidentified influenza virus | oseltamivir |