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Literature summary extracted from

  • Ma, C.; Jin, S.; Wang, J.; Wang, K.; Liu, H.; Wu, K.
    A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy (2016), Anal. Methods, 8, 1989-1994 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.1.78 analysis assay for the detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy. The method is highly sensitive, the T4 PNKP detection limit is 0.01 U/ml Tequatrovirus T4
3.1.3.32 analysis assay for the detection of T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy. The method is highly sensitive, the T4 PNKP detection limit is 0.01 U/ml Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 heparin
-
 Tequatrovirus T4
3.1.3.32 heparin
-
 Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4 P06855
-
-
3.1.3.32 Tequatrovirus T4 P06855 cf. EC 2.7.1.78
-

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.78 additional information
-
 IC50 value is 0.00017 mg/ml, 37°C, pH 7.2 Tequatrovirus T4 heparin
3.1.3.32 additional information
-
 IC50 value is 0.00017 mg/ml, 37°C, pH 7.2 Tequatrovirus T4 heparin