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Literature summary extracted from

  • Adachi, M.; Shimizu, R.; Kato, S.; Oikawa, T.
    The first identification and characterization of a histidine-specific amino acid racemase, histidine racemase from a lactic acid bacterium, Leuconostoc mesenteroides subsp. sake NBRC 102480 (2019), Amino Acids, 51, 331-343 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.24 expression in Escherichia coli Leuconostoc mesenteroides subsp. mesenteroides

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.24 crystal structure in complex with pyridoxal 5'-phosphate at a 2.1 A resolution. Residues Phe44, His286, and Val357 may control the substrate specificity Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.24 8.14
-
 L-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
5.1.1.24 17.26
-
 D-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.1.24 soluble
-
 Leuconostoc mesenteroides subsp. mesenteroides
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.24 D-histidine Leuconostoc mesenteroides subsp. mesenteroides
-
 L-histidine
-
 r
5.1.1.24 D-histidine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 L-histidine
-
 r
5.1.1.24 L-histidine Leuconostoc mesenteroides subsp. mesenteroides
-
 D-histidine
-
 r
5.1.1.24 L-histidine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 D-histidine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.24 Leuconostoc mesenteroides subsp. mesenteroides Q03Z32
-
-
5.1.1.24 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Q03Z32
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.24 D-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides L-histidine
-
 r
5.1.1.24 D-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 L-histidine
-
 r
5.1.1.24 L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides D-histidine
-
 ?
5.1.1.24 L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides D-histidine
-
 r
5.1.1.24 L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 D-histidine
-
 ?
5.1.1.24 L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 D-histidine
-
 r
5.1.1.24 additional information the enzyme is highly specific for histidine. No substrates: L-alanine, L-arginine, L-asparagine, L-glutamate, L-glutamine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, l-phenylalanine, L-proline, L-serine, L-threonine, L-tyrosine, or L-valine Leuconostoc mesenteroides subsp. mesenteroides ?
-
 ?
5.1.1.24 additional information the enzyme is highly specific for histidine. No substrates: L-alanine, L-arginine, L-asparagine, L-glutamate, L-glutamine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, l-phenylalanine, L-proline, L-serine, L-threonine, L-tyrosine, or L-valine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.24 dimer 2 * 42000, calculated from sequence Leuconostoc mesenteroides subsp. mesenteroides

Synonyms

EC Number Synonyms Comment Organism
5.1.1.24 LEUM_0422
-
 Leuconostoc mesenteroides subsp. mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.24 50
-
-
 Leuconostoc mesenteroides subsp. mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.24 40
-
 half-life 9 min Leuconostoc mesenteroides subsp. mesenteroides
5.1.1.24 45
-
 half-life 1.9 min Leuconostoc mesenteroides subsp. mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.24 96.9
-
 L-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
5.1.1.24 185.4
-
 D-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.24 9.5
-
-
 Leuconostoc mesenteroides subsp. mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.24 pyridoxal 5'-phosphate
-
 Leuconostoc mesenteroides subsp. mesenteroides