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Literature summary extracted from

  • Hu, Y.; Yan, X.; Zhang, H.; Liu, J.; Luo, F.; Cui, Y.; Wang, W.; Zhou, Y.
    Cloning and expression of a novel alpha-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68 (2018), AMB Express, 8, 51 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Pichia pastoris GS115 cells Penicillium oxalicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 1.36
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 4.5 and 35°C Penicillium oxalicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Penicillium oxalicum
-
-
-
3.2.1.55 Penicillium oxalicum 68
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 ammonium sulfate precipitation and phenyl Sepharose 6 column chromatography Penicillium oxalicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 mycelium
-
 Penicillium oxalicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O high activity Penicillium oxalicum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O high activity Penicillium oxalicum 68 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-L-glucoside + H2O little activity Penicillium oxalicum 4-nitrophenol + beta-L-glucose
-
 ?
3.2.1.55 4-nitrophenyl beta-L-glucoside + H2O little activity Penicillium oxalicum 68 4-nitrophenol + beta-L-glucose
-
 ?
3.2.1.55 4-nitrophenyl beta-L-xyloside + H2O little activity Penicillium oxalicum 4-nitrophenol + beta-L-xylose
-
 ?
3.2.1.55 additional information the enzyme is specific for cleavage of alpha-1,3-arabinofuranosyl residues, and with the absence of activity towards alpha-1,2-arabinofuranose, alpha-1,5-arabinofuranose, linear alpha-L-1,5-arabinan, xylan, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl alpha-L-galactoside, 4-nitrophenyl beta-L-galactoside and 4-nitrophenyl alpha-L-glucoside Penicillium oxalicum ?
-
 ?
3.2.1.55 additional information the enzyme is specific for cleavage of alpha-1,3-arabinofuranosyl residues, and with the absence of activity towards alpha-1,2-arabinofuranose, alpha-1,5-arabinofuranose, linear alpha-L-1,5-arabinan, xylan, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl alpha-L-galactoside, 4-nitrophenyl beta-L-galactoside and 4-nitrophenyl alpha-L-glucoside Penicillium oxalicum 68 ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O
-
 Penicillium oxalicum ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O
-
 Penicillium oxalicum 68 ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Penicillium oxalicum ?
-
 ?
3.2.1.55 wheat arabinoxylan + H2O
-
 Penicillium oxalicum 68 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 35900, calculated from amino acid sequence Penicillium oxalicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Abf62A
-
 Penicillium oxalicum
3.2.1.55 alpha-1,3-arabinofuranosidase
-
 Penicillium oxalicum
3.2.1.55 exo-alpha-L-arabinofuranosidase
-
 Penicillium oxalicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 35
-
-
 Penicillium oxalicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 25 40 about 60% activity at 25°C, about 80% activity at 30°C, 100% activity at 35°C, about 25% activity at 40°C Penicillium oxalicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 30 45 the enzyme retains about 70% activity after 100 min at 30-35°C and about 10% activity after 100 min at 40-45°C Penicillium oxalicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 0.27
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 4.5 and 35°C Penicillium oxalicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.5
-
-
 Penicillium oxalicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 5.5 more than 50% activity between pH 4.0 and 5.5 Penicillium oxalicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.55 0.2
-
 4-nitrophenyl alpha-L-arabinofuranoside at pH 4.5 and 35°C Penicillium oxalicum