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Literature summary extracted from
- Cloning a novel endo-1,4-beta-D-glucanase gene from Trichoderma virens and heterologous expression in E. coli (2018), AMB Express, 6, 108 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | expressed in Escherichia coli BL21(DE3) cells | Trichoderma virens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | Ni2+ | about 88% residual activity at 2.5 mM | Trichoderma virens |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | Fe3+ | about 150% at 2.5 mM | Trichoderma virens | |
| 3.2.1.74 | Mn2+ | about 160% at 2.5 mM | Trichoderma virens | |
| 3.2.1.74 | additional information | not influenced by Zn2+, Ca2+, Mg2+ and Cu2+ | Trichoderma virens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.74 | Trichoderma virens | A0A288W730 | - |
- |
| 3.2.1.74 | Trichoderma virens ZY-01 | A0A288W730 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | Ni2+ affinity column chromatography | Trichoderma virens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.74 | carboxymethyl cellulose + H2O | - |
Trichoderma virens | ? | - |
? | |
| 3.2.1.74 | carboxymethyl cellulose + H2O | - |
Trichoderma virens ZY-01 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | ? | x * 39000, SDS-PAGE | Trichoderma virens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | endo-1,4-beta-D-glucanase | - |
Trichoderma virens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 40 | - |
- |
Trichoderma virens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 20 | 60 | more than 75% activity between 20 and 75°C | Trichoderma virens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 7 | - |
- |
Trichoderma virens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 6 | 12 | more than 60% activity between pH 6.0 and 12.0 | Trichoderma virens |
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Release 2023.1 (January 2023)
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