BRENDA - Enzyme Database

Characterization of alpha-L-fucosidase and other digestive hydrolases from Biomphalaria glabrata

Perrella, N.; Cantinha, R.; Nakano, E.; Lopes, A.; Acta Trop. 141, 118-127 (2015)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-deoxyfuconojirimycin
-
Biomphalaria glabrata
3.2.1.51
alpha-L-fucose
-
Biomphalaria glabrata
3.2.1.51
Tris
-
Biomphalaria glabrata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.0484
-
4-methylumbelliferyl alpha-L-fucopyranoside
30°C, pH not specified in the publication
Biomphalaria glabrata
3.2.1.51
0.55
-
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH not specified in the publication
Biomphalaria glabrata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
141000
-
gel filtration
Biomphalaria glabrata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Biomphalaria glabrata
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Biomphalaria glabrata
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
749522
Biomphalaria glabrata
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
749522
Biomphalaria glabrata
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
fucoidan + H2O
-
749522
Biomphalaria glabrata
alpha-L-fucose + ?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.8
-
-
Biomphalaria glabrata
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
6.5
8.8
pH 6.5: about 50% of maximal activity, pH 8.8: about 55% of maximal activity
Biomphalaria glabrata
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.00002
-
1-deoxyfuconojirimycin
30°C, pH not specified in the publication
Biomphalaria glabrata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-deoxyfuconojirimycin
-
Biomphalaria glabrata
3.2.1.51
alpha-L-fucose
-
Biomphalaria glabrata
3.2.1.51
Tris
-
Biomphalaria glabrata
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.00002
-
1-deoxyfuconojirimycin
30°C, pH not specified in the publication
Biomphalaria glabrata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.0484
-
4-methylumbelliferyl alpha-L-fucopyranoside
30°C, pH not specified in the publication
Biomphalaria glabrata
3.2.1.51
0.55
-
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH not specified in the publication
Biomphalaria glabrata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
141000
-
gel filtration
Biomphalaria glabrata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Biomphalaria glabrata
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
749522
Biomphalaria glabrata
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
749522
Biomphalaria glabrata
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
fucoidan + H2O
-
749522
Biomphalaria glabrata
alpha-L-fucose + ?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.8
-
-
Biomphalaria glabrata
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.51
6.5
8.8
pH 6.5: about 50% of maximal activity, pH 8.8: about 55% of maximal activity
Biomphalaria glabrata
General Information
EC Number
General Information
Commentary
Organism
3.2.1.51
physiological function
besides its digestive role, the enzyme may also be important in host/parasite interactions
Biomphalaria glabrata
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.2.1.51
physiological function
besides its digestive role, the enzyme may also be important in host/parasite interactions
Biomphalaria glabrata