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Literature summary extracted from

  • Yadava, U.; Vetting, M.W.; Al Obaidi, N.; Carter, M.S.; Gerlt, J.A.; Almo, S.C.
    Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine (2016), Acta Crystallogr. Sect. F, 72, 467-472 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.5.2.11 expressed in Escherichia coli BL21(DE3) cells Agrobacterium vitis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.5.2.11 substrate binding protein Avi_5305 bound to D-glucosamine and D-galactosamine, sitting drop vapor diffusion method, using 30-40% (w/v) PEG 4000 Agrobacterium vitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.5.2.11 ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1] Agrobacterium vitis
-
 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1] Agrobacterium vitis S4
-
 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1] Agrobacterium vitis
-
 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1] Agrobacterium vitis S4
-
 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.5.2.11 Agrobacterium vitis B9K0Q5
-
-
7.5.2.11 Agrobacterium vitis S4 B9K0Q5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.5.2.11 nickel resin column chromatography and Superdex S200 gel filtration Agrobacterium vitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.5.2.11 ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis S4 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis S4 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
7.5.2.11 additional information the enzyme shows specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose Agrobacterium vitis ?
-
 ?
7.5.2.11 additional information the enzyme shows specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose Agrobacterium vitis S4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.5.2.11 ? x * 36800, calculated from amino acid sequence Agrobacterium vitis

Synonyms

EC Number Synonyms Comment Organism
7.5.2.11 Avi_5305 solute binding protein Agrobacterium vitis
7.5.2.11 Pfam13407
-
 Agrobacterium vitis