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Literature summary extracted from

  • Liu, T.; Zhu, W.; Wang, J.; Zhou, Y.; Duan, Y.; Qu, M.; Yang, Q.
    The deduced role of a chitinase containing two nonsynergistic catalytic domains (2018), Acta Crystallogr. Sect. D, 74, 30-40 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Pichia pastoris GS115 cells Ostrinia furnacalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 hanging drop vapor diffusion method, using 200 mM ammonium sulfate, 100 mM bis-Tris pH 6.5, 20% (w/v) PEG 3350 for domain GH18A, and 200 mM trisodium citrate dihydrate pH 8.1, 20% (w/v) PEG 3350 for domain GH18B Ostrinia furnacalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 E217L domain GH18A active site mutant Ostrinia furnacalis
3.2.1.14 E647L domain GH18B active site mutant Ostrinia furnacalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 single-chained chitin + H2O Ostrinia furnacalis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Ostrinia furnacalis U5LUV7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 HisTrap column chromatography and Resource S column chromatography Ostrinia furnacalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 cuticle
-
 Ostrinia furnacalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 ethylene glycol chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.14 hexa-N-acetyl-chitohexaose + H2O highest activity Ostrinia furnacalis 2 tri-N-acetyl-chitotriose
-
 ?
3.2.1.14 additional information the enzyme is inactive towards tri-N-acetyl-chitotriose and insoluble chitin substrates like alpha-chitin, beta-chitin, and colloidal chitin Ostrinia furnacalis ?
-
 ?
3.2.1.14 penta-N-acetylchitopentaose + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.14 single-chained chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
3.2.1.14 tetra-N-acetyl-chitotetraose + H2O
-
 Ostrinia furnacalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChtIII
-
 Ostrinia furnacalis
3.2.1.14 group III chitinase
-
 Ostrinia furnacalis