Literature summary extracted from
Hernandez Lozada, N.J.; Lai, R.Y.; Simmons, T.R.; Thomas, K.A.; Chowdhury, R.; Maranas, C.D.; Pfleger, B.F.
Highly active C8-acyl-ACP thioesterase variant isolated by a synthetic selection strategy (2018), ACS Synth. Biol., 7, 2205-2215 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.2.14 |
expressed in Escherichia coli DELTAlipB cells |
Cuphea palustris |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.2.14 |
A59S/K296R |
the mutant exhibits 4fold higher enzymatic activities than the wild type enzyme in vivo |
Cuphea palustris |
3.1.2.14 |
DELTAA54/N28S/I65M |
the mutant exhibits much higher enzymatic activities than the wild type enzyme in vivo |
Cuphea palustris |
3.1.2.14 |
H224A |
catalytically inactive |
Cuphea palustris |
3.1.2.14 |
K15E/S207T |
the mutant exhibits wild type activity |
Cuphea palustris |
3.1.2.14 |
L251M/L265I |
the mutant exhibits wild type activity |
Cuphea palustris |
3.1.2.14 |
M29T/T117S/Q163L |
the mutant exhibits wild type activity |
Cuphea palustris |
3.1.2.14 |
R261S |
the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo |
Cuphea palustris |
3.1.2.14 |
W17R/T204S |
the mutant exhibits higher enzymatic activities than the wild type enzyme in vivo |
Cuphea palustris |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.2.14 |
0.00592 |
- |
octanoyl-[acyl-carrier protein] |
at pH 7.4, temperature not specified in the publication |
Cuphea palustris |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.2.14 |
octanoyl-[acyl-carrier protein] + H2O |
Cuphea palustris |
specific substrate |
[acyl-carrier protein] + octanoate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.2.14 |
Cuphea palustris |
Q39554 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.2.14 |
Ni-NTA column chromatography |
Cuphea palustris |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.2.14 |
octanoyl-[acyl-carrier protein] + H2O |
specific substrate |
Cuphea palustris |
[acyl-carrier protein] + octanoate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.2.14 |
C8-acyl-ACP thioesterase |
- |
Cuphea palustris |
3.1.2.14 |
FatB1 thioesterase |
- |
Cuphea palustris |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.2.14 |
1.14 |
- |
octanoyl-[acyl-carrier protein] |
at pH 7.4, temperature not specified in the publication |
Cuphea palustris |
|
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.2.14 |
193.3 |
- |
octanoyl-[acyl-carrier protein] |
at pH 7.4, temperature not specified in the publication |
Cuphea palustris |
|