EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.62 | expressed in Escherichia coli BL21(DE3) cells | Rhodococcus sp. M-777 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.62 | D311Y | the mutant enzyme is characterized by a lowered KM value for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside and increased transglycosylation when using aliphatic 1,3-diols or alcohols bearing a delta-hydroxy ketone function as acceptors. The transglycosylation/hydrolysis ratio in reactions catalyzed by the mutant is completely inversed and weak secondary hydrolysis is postponed, thus providing the basis for high transglycosylation yields (between 68 and 93%) | Rhodococcus sp. M-777 |
3.2.1.62 | D311Y | the mutant shows increased catalytic efficiency for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside compared to the wild type enzyme. The transglycosylation ability of the mutant is improved in comparison to the wild type enzyme | Rhodococcus sp. M-777 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 7 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D311Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 22.4 | - |
2-chloro-4-nitrophenyl beta-cellobioside | wild type enzyme, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 22.7 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D314Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.62 | Rhodococcus sp. M-777 | O33853 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.62 | TALON metal affinity resin column chromatography | Rhodococcus sp. M-777 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.62 | 2-chloro-4-nitrophenyl beta-cellobioside + H2O | poorly recognized substrate | Rhodococcus sp. M-777 | 2-chloro-4-nitrophenol + beta-cellobiose | - |
ir | |
3.2.1.62 | alpha-cellobioside + 1-hydroxyoctan-4-one | transglycosylation reaction | Rhodococcus sp. M-777 | 4-oxooct-1-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside | - |
r | |
3.2.1.62 | alpha-cellobioside + 5-hydroxypentan-2-one | transglycosylation reaction | Rhodococcus sp. M-777 | 2-oxopent-5-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside | - |
r | |
3.2.1.62 | alpha-cellobioside + octane-1,3-diol | transglycosylation reaction | Rhodococcus sp. M-777 | 3-hydroxyoct-1-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside + 1-(2-hydroxy)ethylhex-1-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside | - |
r | |
3.2.1.62 | alpha-cellobioside + pentane-1,3-diol | transglycosylation reaction | Rhodococcus sp. M-777 | 3-hydroxypent-1-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside + 1-ethyl(3-hydroxy)prop-1-yl beta-D-glucopyranosyl(1->4)-beta-D-glucopyranoside | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.62 | EGC | - |
Rhodococcus sp. M-777 |
3.2.1.62 | endo-glycoceramidase II | - |
Rhodococcus sp. M-777 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 2.9 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D311Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 3.7 | - |
2-chloro-4-nitrophenyl beta-cellobioside | wild type enzyme, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 5.4 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D314Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.62 | 0.17 | - |
2-chloro-4-nitrophenyl beta-cellobioside | wild type enzyme, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 0.24 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D314Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 | |
3.2.1.62 | 0.42 | - |
2-chloro-4-nitrophenyl beta-cellobioside | mutant enzyme D311Y, hydrolysis reaction, at pH 4.0 and 37°C | Rhodococcus sp. M-777 |