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Literature summary extracted from

  • Sinha, S.; Chand, S.; Tripathi, P.
    Enzymatic production of glucosamine and chitooligosaccharides using newly isolated exo-beta-D-glucosaminidase having transglycosylation activity (2016), 3 Biotech, 6, 13 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.165 EDTA 120% activity at 1 mM Aspergillus fumigatus
3.2.1.165 NaCl the optimum NaCl concentration for the enzyme is 250 mM Aspergillus fumigatus

General Stability

EC Number General Stability Organism
3.2.1.165 electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme retains more than 50% of its catalytic activity after 10 batches of uses in aqueous solution Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.165 2-Hydroxy-5-nitrobenzyl bromide 65% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 4-chloromercuribenzoate 88% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Ba2+ 98% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Ca2+ 88% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Cd2+ 90% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Co2+ 32% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Cu2+ 98% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 dithiothreitol 90% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Fe2+ 87% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Hg2+ 76% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 iodoacetate 93% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Mg2+ 95% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Mn2+ 98% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 N-ethylmaleimide 66% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Ni2+ 87% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Triton X-100 88% residual activity at 1 mM Aspergillus fumigatus
3.2.1.165 Tween 20 76% residual activity at 1 mM Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.165 Zn2+ 102% activity at 1 mM Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.165 chitosan + H2O Aspergillus fumigatus
-
 D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O Aspergillus fumigatus IIT-004
-
 D-glucosamine + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Aspergillus fumigatus
-
-
-
3.2.1.165 Aspergillus fumigatus IIT-004
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 acetone fractionation, DEAE Sepharose column chromatography, CM-Sepharose column chromatography, and Superdex 200 gel filtration Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.165 mycelium
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.165 0.5
-
 crude enzyme, at pH 5.5 and 40°C Aspergillus fumigatus
3.2.1.165 13
-
 after 26fold purification, at pH 5.5 and 40°C Aspergillus fumigatus

Storage Stability

EC Number Storage Stability Organism
3.2.1.165 25°C, electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme, 30 days, 40% loss of activity Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitin + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.165 chitin + H2O
-
 Aspergillus fumigatus IIT-004 ?
-
 ?
3.2.1.165 chitosan + H2O
-
 Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 70% degree of acetylation, 43% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 80% degree of acetylation, 68% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O
-
 Aspergillus fumigatus IIT-004 D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus IIT-004 D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus IIT-004 D-glucosamine + ?
-
 ?
3.2.1.165 colloidal chitosan + H2O 56% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 crab shell chitosan + H2O more than 90% degree of acetylation, 100% activity Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 D-glucosamine + N-acetyl-D-glucosamine 15% (w/v) ammonium sulfate is optimum for catalyzing transglycosylation reaction Aspergillus fumigatus chitodimer
-
 ?
3.2.1.165 glycol chitosan + H2O 12% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?
3.2.1.165 additional information no activity with flaked chitin, colloidal chitin and cellulose Aspergillus fumigatus ?
-
 ?
3.2.1.165 additional information no activity with flaked chitin, colloidal chitin and cellulose Aspergillus fumigatus IIT-004 ?
-
 ?
3.2.1.165 shrimp shell chitosan + H2O more than 90% degree of acetylation, 57% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.165 ? x * 64000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Aspergillus fumigatus
3.2.1.165 exochitosanase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 40
-
 free enzyme Aspergillus fumigatus
3.2.1.165 50
-
 electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme Aspergillus fumigatus
3.2.1.165 50
-
 optimum for transglycosylation reaction Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.165 50
-
 the enzyme remains stable at up to 50°C for 32 h Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.165 3000
-
 crab shell chitosan at pH 5.5 and 40°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 5.5
-
 free enzyme Aspergillus fumigatus
3.2.1.165 5.8
-
 electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.165 Aspergillus fumigatus isoelectric focusing
-
 6.5