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Literature summary extracted from

  • Zerbe, P.; Bohlmann, J.
    Plant diterpene synthases exploring modularity and metabolic diversity for bioengineering (2015), Trends Biotechnol., 33, 419-428 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.141 synthesis as biotechnological production systems for sesquiterpenoids, a number of diterpenoid engineering platforms are described that use plant diTPSs (and in some cases P450s) to produce, for example, the fragrance precursors sclareol and cis-abienol in the prefume industry. Sclareol from clary sage (Salvia sclarea) is used as a precursor for ambroxide fragrance and fixatives in perfume manufacture Salvia sclarea

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.141 additional information usage of fed-batch fermentation along with an organic solvent overlay for in situ product removal to develop an industrial-scale platform for sclareol production Salvia sclarea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.141 (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O Salvia sclarea
-
 sclareol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.141 Salvia sclarea K4HYB0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.141 (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O
-
 Salvia sclarea sclareol + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.141 SsSS
-
 Salvia sclarea

General Information

EC Number General Information Comment Organism
4.2.3.141 evolution in angiosperms, class II diTPSs that enable water quenching of the labda-13-en-8-yl carbocation are found in six different plant families, phylogenetic tree, overview Salvia sclarea
4.2.3.141 physiological function in nature, diterpene synthase (diTPS) enzymes are essential for generating diverse diterpene hydrocarbon scaffolds. Major commercial diterpenoids include sclareol from clary sage (Salvia sclarea) used as a precursor for ambroxide fragrance and fixatives in perfume manufacture. In angiosperms, class II diTPSs that enable water quenching of the labda-13-en-8-yl carbocation are found in six different plant families, these class II diTPSs can pair with class I enzymes, converting oxygenated prenyl diphosphates through ionization with or without secondary cyclization or additional hydroxylation to afford diterpene alcohols, including manoyl oxide, sclareol and cis-abienol, that are of interest in the fragrance industry Salvia sclarea