BRENDA - Enzyme Database

The crystal structure of D-xylonate dehydratase reveals functional features of enzymes from the Ilv/ED dehydratase family

Rahman, M.M.; Andberg, M.; Koivula, A.; Rouvinen, J.; Hakulinen, N.; Sci. Rep. 8, 865 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.82
heterologously expressed in Escherichia coli
Caulobacter vibrioides
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.82
the crystal structure of D-xylonate dehydratase from Caulobacter crescentus is described at 2.7 A resolution and it is compares with other available enzyme structures from the IlvD/EDD protein family
Caulobacter vibrioides
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.82
Mg2+
each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.82
Caulobacter vibrioides
Q9A9Z2
-
-
4.2.1.82
Caulobacter vibrioides ATCC 19089
Q9A9Z2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.82
-
Caulobacter vibrioides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.82
D-xylonate
-
749370
Caulobacter vibrioides
2-dehydro-3-deoxy-D-xylonate + H2O
-
-
-
?
4.2.1.82
D-xylonate
-
749370
Caulobacter vibrioides ATCC 19089
2-dehydro-3-deoxy-D-xylonate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.82
tetramer
crystallographic data, each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.82
30
-
assay at
Caulobacter vibrioides
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.82
8
-
assay at
Caulobacter vibrioides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.82
[2Fe-2S]-center
each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.82
heterologously expressed in Escherichia coli
Caulobacter vibrioides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.82
[2Fe-2S]-center
each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.82
the crystal structure of D-xylonate dehydratase from Caulobacter crescentus is described at 2.7 A resolution and it is compares with other available enzyme structures from the IlvD/EDD protein family
Caulobacter vibrioides
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.82
Mg2+
each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.82
-
Caulobacter vibrioides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.82
D-xylonate
-
749370
Caulobacter vibrioides
2-dehydro-3-deoxy-D-xylonate + H2O
-
-
-
?
4.2.1.82
D-xylonate
-
749370
Caulobacter vibrioides ATCC 19089
2-dehydro-3-deoxy-D-xylonate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.82
tetramer
crystallographic data, each monomer is composed of two domains in which the N-terminal domain forms a binding site for a [2Fe-2S] cluster and a Mg2+ ion
Caulobacter vibrioides
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.82
30
-
assay at
Caulobacter vibrioides
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.82
8
-
assay at
Caulobacter vibrioides