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Literature summary extracted from

  • Orelle, C.; Durmort, C.; Mathieu, K.; Duchene, B.; Aros, S.; Fenaille, F.; Andre, F.; Junot, C.; Vernet, T.; Jault, J.M.
    A multidrug ABC transporter with a taste for GTP (2018), Sci. Rep., 8, 2309 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 expressed in Escherichia coli C41(DE3) cells Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.2 orthovanadate complete inhibition at 4 mM Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.2 2.64
-
 GTP at pH 7.4 and 37°C Streptococcus pneumoniae
7.6.2.2 2.83
-
 ATP at pH 7.4 and 37°C Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.2 Ni-NTA resin column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + ciprofloxacin[side 1]
-
 Streptococcus pneumoniae ADP + phosphate + ciprofloxacin[side 2]
-
 ?
7.6.2.2 ATP + H2O + doxorubicin[side 1]
-
 Streptococcus pneumoniae ADP + phosphate + doxorubicin[side 2]
-
 ?
7.6.2.2 ATP + H2O + Hoechst33342[side 1]
-
 Streptococcus pneumoniae ADP + phosphate + Hoechst33342[side 2]
-
 ?
7.6.2.2 ATP + H2O + norfloxacin[side 1]
-
 Streptococcus pneumoniae ADP + phosphate + norfloxacin[side 2]
-
 ?
7.6.2.2 GTP + H2O + ciprofloxacin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + ciprofloxacin[side 2]
-
 ?
7.6.2.2 GTP + H2O + doxorubicin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + doxorubicin[side 2]
-
 ?
7.6.2.2 GTP + H2O + Hoechst33342[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity. Hoechst 33342 is 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole Streptococcus pneumoniae GDP + phosphate + Hoechst33342[side 2]
-
 ?
7.6.2.2 GTP + H2O + norfloxacin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + norfloxacin[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.2 heterodimer
-
 Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
7.6.2.2 multidrug ABC transporter
-
 Streptococcus pneumoniae
7.6.2.2 PatA/PatB
-
 Streptococcus pneumoniae